Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,385,857 C→T *91* (TAG→TAA yhcO ← putative barnase inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,385,8570CT100.0% 46.0 / NA 16*91* (TAG→TAAyhcOputative barnase inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/9);  total (7/9)

TCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAT  >  NC_000913/3385756‑3385973
                                                                                                     |                                                                                                                    
tCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                 <  1:280390/139‑1 (MQ=255)
                  gCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                     >  1:71227/1‑117 (MQ=255)
                  gCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                     <  2:71227/117‑1 (MQ=255)
                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTaaaa                                                                                                    >  1:816096/1‑73 (MQ=255)
                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTaaaa                                                                                                    <  2:816096/73‑1 (MQ=255)
                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAGATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                           >  1:516338/1‑114 (MQ=255)
                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                             <  1:818899/112‑1 (MQ=255)
                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                             >  2:818899/1‑112 (MQ=255)
                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACCAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                           <  2:516338/114‑1 (MQ=255)
                                                   cATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAcgcg                              <  1:780449/139‑1 (MQ=255)
                                                            gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGc                                                                                           <  1:554452/69‑1 (MQ=255)
                                                            gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGc                                                                                           >  2:554452/1‑69 (MQ=255)
                                                             tCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTcc                    >  1:625213/1‑139 (MQ=255)
                                                                             ccGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCa    <  2:732125/139‑1 (MQ=255)
                                                                              cGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAATCGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCaa   >  2:535029/1‑139 (MQ=255)
                                                                               ggTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAt  <  1:535029/139‑1 (MQ=255)
                                                                                                     |                                                                                                                    
TCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAT  >  NC_000913/3385756‑3385973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: