| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,440,950 | G→A | T27I (ACC→ATC) | rpoA ← | RNA polymerase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,440,950 | 0 | G | A | 100.0% | 106.7 / NA | 34 | T27I (ACC→ATC) | rpoA | RNA polymerase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/15); total (19/15) | |||||||||||
TACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGA > NC_000913/3440827‑3441074 | tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgtgcg > 1:834494/1‑139 (MQ=255) gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACt > 1:96977/1‑139 (MQ=255) gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACt > 2:625061/1‑139 (MQ=255) agtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCa < 2:310163/139‑1 (MQ=255) gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc > 2:401977/1‑139 (MQ=255) gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc > 1:807457/1‑139 (MQ=255) ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt < 2:96977/139‑1 (MQ=255) ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt < 2:807457/139‑1 (MQ=255) tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGa > 2:236020/1‑139 (MQ=255) tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGa > 2:321469/1‑139 (MQ=255) cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg < 2:345875/139‑1 (MQ=255) ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg > 1:302740/1‑139 (MQ=255) cGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca < 1:401977/139‑1 (MQ=255) agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCt < 2:302740/139‑1 (MQ=255) cAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGc > 1:820176/1‑139 (MQ=255) gAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAt < 2:820176/139‑1 (MQ=255) cgcAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt < 2:109118/82‑1 (MQ=255) cgcAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt > 1:109118/1‑82 (MQ=255) aGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTACGTCGAACTCACTTGCTCGATATCAACCAGgcg > 2:212509/1‑95 (MQ=255) aGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTACGTCGAACTCACTTGCTCGATATCAACCAGgcg < 1:212509/95‑1 (MQ=255) agagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg > 2:329296/1‑83 (MQ=255) agagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg < 1:329296/83‑1 (MQ=255) agagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAg > 2:129018/1‑139 (MQ=255) ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt > 2:280103/1‑60 (MQ=255) ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt < 1:280103/60‑1 (MQ=255) ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt > 2:598236/1‑139 (MQ=255) aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt < 1:67578/55‑1 (MQ=255) aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt > 2:67578/1‑55 (MQ=255) aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAg > 2:118157/1‑139 (MQ=255) aGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg > 1:677946/1‑70 (MQ=255) aGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg < 2:677946/70‑1 (MQ=255) cGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc > 1:772755/1‑44 (MQ=255) cGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc < 2:772755/44‑1 (MQ=255) ctAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTcga < 1:118157/139‑1 (MQ=255) | TACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGA > NC_000913/3440827‑3441074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |