Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,506,024	T→C	intergenic (‑4/+8)	yhfS ← / ← yhfT	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein/inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,506,024	0	T	C	100.0%	61.9 / NA	18	intergenic (‑4/+8)	yhfS/yhfT	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/11);  total (7/11)

AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTGAG  >  NC_000913/3505896‑3506148                                                                                                                                 |                                                                                                                             aaGCCTAAATCACCGCCAGTAA‑AAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTaa                                                                                                                   >  2:274606/1‑139 (MQ=255)                       gAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGc                                                                                              <  1:327085/139‑1 (MQ=255)                       aaaaCTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGc                                                                                              <  1:274606/138‑1 (MQ=255)                            tCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTa                                                                                         >  1:558357/1‑139 (MQ=255)                                                     tGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTg                                                                >  2:416815/1‑139 (MQ=255)                                                                           cTTTTGCTGCGCCTCAATAAACGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTg                                          >  2:493371/1‑139 (MQ=255)                                                                                               tCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTcagca                                                                           <  1:326300/86‑1 (MQ=255)                                                                                               tCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTcagca                                                                           >  2:326300/1‑86 (MQ=255)                                                                                               tCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                      <  2:181199/139‑1 (MQ=255)                                                                                               tCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                      <  2:558357/139‑1 (MQ=255)                                                                                               tCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                      <  1:493371/139‑1 (MQ=255)                                                                                                 gTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGgaacgaa                                                                                                        >  1:640009/1‑55 (MQ=255)                                                                                                 gTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGgaacgaa                                                                                                        <  2:640009/55‑1 (MQ=255)                                                                                                            cAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGAtt         <  1:396717/139‑1 (MQ=255)                                                                                                                 gAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTg    <  2:828994/139‑1 (MQ=255)                                                                                                                   aaCGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTGAg  <  1:385338/139‑1 (MQ=255)                                                                                                                         ttCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                      <  1:702755/113‑1 (MQ=255)                                                                                                                         ttCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                      >  2:702755/1‑113 (MQ=255)                                                                                                                                 |                                                                                                                             AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTGAG  >  NC_000913/3505896‑3506148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑