Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,531,180 G→A A420A (GCC→GCT)  yhgE ← DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,531,1800GA100.0% 139.8 / NA 46A420A (GCC→GCT) yhgEDUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/24);  total (22/24)

ATTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCGGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATA  >  NC_000913/3531070‑3531315
                                                                                                              |                                                                                                                                       
aTTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                             <  1:697638/139‑1 (MQ=255)
aTTAACACATTATCGGCTAATACTTTTTCCGATACCTGTGGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCTAGCgcag                                                                                                             <  1:512338/139‑1 (MQ=255)
       caTTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTcc                                                                                                      >  1:302664/1‑139 (MQ=255)
           aTCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                             >  1:837056/1‑128 (MQ=255)
           aTCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                             <  2:837056/128‑1 (MQ=255)
                  aaTACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCCTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGc                                                                                           >  1:307581/1‑139 (MQ=255)
                        ttttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGAc                                                                                              >  2:262681/1‑130 (MQ=255)
                        ttttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGAc                                                                                              <  1:262681/130‑1 (MQ=255)
                         tttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTc                                                                                    >  1:372217/1‑139 (MQ=255)
                          ttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCg                                                                                   <  1:728433/139‑1 (MQ=255)
                                    tGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCCTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGa                                                                         <  2:307581/139‑1 (MQ=255)
                                       tGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAg                                                                      >  2:702429/1‑139 (MQ=255)
                                                 gTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCg                                                                                            <  2:774657/107‑1 (MQ=255)
                                                 gTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCg                                                                                            >  1:774657/1‑107 (MQ=255)
                                                   tGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcagc                                                                                                            >  2:757937/1‑89 (MQ=255)
                                                   tGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcagc                                                                                                            <  1:757937/89‑1 (MQ=255)
                                                   tGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTg                                                          >  2:804465/1‑139 (MQ=255)
                                                      tctcCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                             <  1:153141/85‑1 (MQ=255)
                                                      tctcCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                             >  2:153141/1‑85 (MQ=255)
                                                      tctcCATCTAACGCCATCAGCAGATCCCGCGAGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACATGGTATGACg                                                       >  2:775077/1‑139 (MQ=255)
                                                          cATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCTATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTc                                                            >  2:395800/1‑130 (MQ=255)
                                                          cATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCTATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTc                                                            <  1:395800/130‑1 (MQ=255)
                                                                 cGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCg                                            >  1:652772/1‑139 (MQ=255)
                                                                 cGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCg                                            >  2:243154/1‑139 (MQ=255)
                                                                    cATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcagca                                                                                                           <  1:269588/73‑1 (MQ=255)
                                                                    cATCAGCAGATCACGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcagca                                                                                                           >  2:269588/1‑73 (MQ=255)
                                                                     aTCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGctct                                        <  1:267959/139‑1 (MQ=255)
                                                                      tCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGc                                                                                <  2:405263/98‑1 (MQ=255)
                                                                      tCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGc                                                                                >  1:405263/1‑98 (MQ=255)
                                                                            gATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAAcg                                 <  1:211295/139‑1 (MQ=255)
                                                                             aTCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTc                                                                                                                     <  2:373488/54‑1 (MQ=255)
                                                                             aTCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTc                                                                                                                     >  1:373488/1‑54 (MQ=255)
                                                                             aTCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAg                                                                                 >  1:517481/1‑90 (MQ=255)
                                                                             aTCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAg                                                                                 <  2:517481/90‑1 (MQ=255)
                                                                              tCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATCGCGACCGCTCTGCTCGAGCATGTAGATAg                                                                      >  2:352172/1‑100 (MQ=255)
                                                                              tCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAg                                                                      <  1:352172/100‑1 (MQ=255)
                                                                                   cgcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACt                                                                                                                      >  2:82668/1‑47 (MQ=255)
                                                                                   cgcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACt                                                                                                                      <  1:82668/47‑1 (MQ=255)
                                                                                    gcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCatat                         >  1:400661/1‑139 (MQ=255)
                                                                                           tCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGtt                  <  1:804465/139‑1 (MQ=255)
                                                                                             gggTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCa                <  1:94036/139‑1 (MQ=255)
                                                                                                 cTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCt            <  2:372217/139‑1 (MQ=255)
                                                                                                 cTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCt            <  2:87598/139‑1 (MQ=255)
                                                                                                    tCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAg                                                                                 <  2:50424/67‑1 (MQ=255)
                                                                                                    tCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAg                                                                                 >  1:50424/1‑67 (MQ=255)
                                                                                                           cccAGCATCGCGTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCGTCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATa  <  1:547698/139‑1 (MQ=255)
                                                                                                              |                                                                                                                                       
ATTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCGGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATA  >  NC_000913/3531070‑3531315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑