Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,695,997	C→T	*63* (TAG→TAA)	yhjR ←	DUF2629 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,695,997	0	C	T	100.0%	206.9 / NA	64	*63* (TAG→TAA)	yhjR	DUF2629 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/29);  total (35/29)

ACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATC  >  NC_000913/3695879‑3696125                                                                                                                       |                                                                                                                                 accaccAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAActc                                                                                                              >  2:421160/1‑139 (MQ=255)                        cATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTa                                                                                       >  2:241797/1‑139 (MQ=255)                           ttGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATg                                                                                    >  1:751239/1‑139 (MQ=255)                                   aCCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                           >  2:336738/1‑108 (MQ=255)                                   aCCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                           <  1:336738/108‑1 (MQ=255)                                     cAGGCTAATGCGGCGGTGTTCGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTGCGGCCATGCACCAATCCCTTACTTTTGTCTCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCaa                                                                          >  2:789238/1‑139 (MQ=255)                                        gCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTg                                                                       <  1:241797/139‑1 (MQ=255)                                             tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAg                                                                                              >  2:835601/1‑111 (MQ=255)                                             tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAg                                                                                              <  1:835601/111‑1 (MQ=255)                                                  cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTgg                                                             >  1:144451/1‑139 (MQ=255)                                                  cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCATTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTgg                                                             >  1:520206/1‑139 (MQ=255)                                                            tgttgt‑CCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                  <  2:605374/139‑1 (MQ=255)                                                             gttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                  <  1:555588/139‑1 (MQ=255)                                                             gttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                  <  2:248528/139‑1 (MQ=255)                                                             gttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                  <  2:520206/139‑1 (MQ=255)                                                              ttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                 <  1:120723/139‑1 (MQ=255)                                                                 tCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATaa                                              >  2:819538/1‑139 (MQ=255)                                                                                  aCCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGa                             >  2:209462/1‑139 (MQ=255)                                                                                            aTCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                 <  2:730767/61‑1 (MQ=255)                                                                                            aTCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                 >  1:730767/1‑61 (MQ=255)                                                                                              cccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGTGTTTTTATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                  <  1:561511/106‑1 (MQ=255)                                                                                              cccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                  >  2:561511/1‑106 (MQ=255)                                                                                              cccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCtt                 >  2:831342/1‑139 (MQ=255)                                                                                               ccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCttt                <  1:366603/139‑1 (MQ=255)                                                                                                  gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaa                                                                                                                  <  1:637319/38‑1 (MQ=37)                                                                                                  gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaa                                                                                                                  >  2:637319/1‑38 (MQ=37)                                                                                                  gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAAct                                                                                                               >  1:252599/1‑41 (MQ=255)                                                                                                  gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAAct                                                                                                               <  2:252599/41‑1 (MQ=255)                                                                                                  gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                 >  1:332936/1‑103 (MQ=255)                                                                                                  gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                 <  2:332936/103‑1 (MQ=255)                                                                                                  gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAgg                                   <  2:379340/117‑1 (MQ=255)                                                                                                  gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAgg                                   >  1:379340/1‑117 (MQ=255)                                                                                                  gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAAc             >  2:837378/1‑139 (MQ=255)                                                                                                  gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAAc             >  1:568630/1‑139 (MQ=255)                                                                                                    actgCCATTCACCCATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCCCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGc           <  2:554458/136‑1 (MQ=255)                                                                                                    aCGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                           >  2:217981/1‑43 (MQ=255)                                                                                                    aCGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                           <  1:217981/43‑1 (MQ=255)                                                                                                    aCGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAGTATCAGGCAGTGAAAATGCCTGCTTTAACGc           >  2:186648/1‑139 (MQ=255)                                                                                                    aCGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGc           <  1:831342/139‑1 (MQ=255)                                                                                                    aCGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGc           >  1:763396/1‑139 (MQ=255)                                                                                                      ggCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAg                                                                                              >  2:582574/1‑54 (MQ=255)                                                                                                      ggCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAg                                                                                              <  1:582574/54‑1 (MQ=255)                                                                                                      ggCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCca         <  2:234114/139‑1 (MQ=255)                                                                                                       gCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                 <  1:830774/98‑1 (MQ=255)                                                                                                       gCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                 >  2:830774/1‑98 (MQ=255)                                                                                                        ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                  <  1:398888/96‑1 (MQ=255)                                                                                                        ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                  >  2:398888/1‑96 (MQ=255)                                                                                                        ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTaa              >  2:204673/1‑132 (MQ=255)                                                                                                        ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTaa              <  1:204673/132‑1 (MQ=255)                                                                                                        ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCcaca       >  2:171775/1‑139 (MQ=255)                                                                                                          aTTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTggg                                                            <  2:502765/84‑1 (MQ=255)                                                                                                          aTTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTggg                                                            >  1:502765/1‑84 (MQ=255)                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                           >  1:196752/1‑36 (MQ=37)                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                           <  2:196752/36‑1 (MQ=37)                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                 <  1:78608/46‑1 (MQ=255)                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                 >  2:78608/1‑46 (MQ=255)                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                  >  2:407692/1‑93 (MQ=255)                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                  <  1:407692/93‑1 (MQ=255)                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCca         >  1:15102/1‑134 (MQ=255)                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCca         <  2:15102/134‑1 (MQ=255)                                                                                                           ttCACCAATCCCTTACTTTTGTTGCGCAAACTATGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAata    >  1:664846/1‑139 (MQ=255)                                                                                                             cACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATc  >  2:53135/1‑139 (MQ=255)                                                                                                                  aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTaa              >  2:66716/1‑122 (MQ=255)                                                                                                                  aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTaa              <  1:66716/122‑1 (MQ=255)                                                                                                                       |                                                                                                                                 ACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATC  >  NC_000913/3695879‑3696125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑