Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,781,017 G→A *397* (TAG→TAA)  lldD → L‑lactate dehydrogenase, FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0170GA100.0% 173.9 / NA 55*397* (TAG→TAA) lldDL‑lactate dehydrogenase, FMN‑linked
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

AGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAG  >  NC_000913/3780887‑3781139
                                                                                                                                  |                                                                                                                          
agaTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgc                                                                                                                    <  2:324524/139‑1 (MQ=255)
  aTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgcgc                                                                                                                  >  1:158328/1‑139 (MQ=255)
     aaaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGcccc                                                                                                               <  1:243213/139‑1 (MQ=255)
      aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGccccc                                                                                                              <  2:158328/139‑1 (MQ=255)
       aGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                             <  1:795922/139‑1 (MQ=255)
         tGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCa                                                                                                           >  2:440155/1‑139 (MQ=255)
            cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCAccc                                                                                                        >  2:548485/1‑139 (MQ=255)
                     tGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCctct                                                                                               <  1:440155/139‑1 (MQ=255)
                                           cGAAATTACGCAAGATTCGCTGGTGCTGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                         <  1:291980/139‑1 (MQ=255)
                                           cGAAATTACGCAAGATTCGCTGGTGCAGGTGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGCAATGCGGCATAATCGTTTGCGccccc                                                                                                              >  1:354883/1‑102 (MQ=255)
                                           cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCgccgcc                                                                                                              <  2:354883/102‑1 (MQ=255)
                                               aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCGCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                     <  1:548485/139‑1 (MQ=255)
                                                ttACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCACCCTCAGGGAGAGGGGACCGTTCGgcg                                                                    <  1:542626/139‑1 (MQ=255)
                                                   cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgcg                                                                                                                   <  1:564329/89‑1 (MQ=255)
                                                   cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgcg                                                                                                                   >  2:564329/1‑89 (MQ=255)
                                                         aTTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatgta                                                           <  2:467390/139‑1 (MQ=255)
                                                                  tGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCct                                                                                                 <  1:343093/92‑1 (MQ=255)
                                                                  tGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCct                                                                                                 >  2:343093/1‑92 (MQ=255)
                                                                    cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc                                                >  2:157861/1‑139 (MQ=255)
                                                                           tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCt                                                                                                       <  1:292196/77‑1 (MQ=255)
                                                                           tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCt                                                                                                       >  2:292196/1‑77 (MQ=255)
                                                                           tGGGTAAAGAGTTGCCTGCGACACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACg                                         >  1:568886/1‑139 (MQ=255)
                                                                            gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCAccc                                                                                                        >  2:383137/1‑75 (MQ=255)
                                                                            gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCAccc                                                                                                        <  1:383137/75‑1 (MQ=255)
                                                                            gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                    >  1:329877/1‑111 (MQ=255)
                                                                            gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                    <  2:329877/111‑1 (MQ=255)
                                                                            gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                    <  2:336950/111‑1 (MQ=255)
                                                                            gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                    >  1:336950/1‑111 (MQ=255)
                                                                            gggTAAAGAGTTGCCTGC‑GCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                         <  2:181807/105‑1 (MQ=255)
                                                                            gggTAAAGAGTTGCCTGC‑GCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                         >  1:181807/1‑105 (MQ=255)
                                                                                       tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTaa                                                                                                     >  2:438509/1‑67 (MQ=255)
                                                                                       tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTaa                                                                                                     <  1:438509/67‑1 (MQ=255)
                                                                                       tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGgaga                                                                                   >  2:315941/1‑85 (MQ=255)
                                                                                       tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGgaga                                                                                   <  1:315941/85‑1 (MQ=255)
                                                                                       tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGga                                                                                     >  2:195487/1‑83 (MQ=255)
                                                                                       tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGga                                                                                     <  1:195487/83‑1 (MQ=255)
                                                                                        gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                             >  1:565546/1‑58 (MQ=255)
                                                                                        gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                             <  2:565546/58‑1 (MQ=255)
                                                                                           tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                     <  1:356729/95‑1 (MQ=255)
                                                                                           tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                     >  2:356729/1‑95 (MQ=255)
                                                                                                        cccATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc             >  2:18296/1‑138 (MQ=255)
                                                                                                        cccATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc             <  1:18296/138‑1 (MQ=255)
                                                                                                               cGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg     >  1:81203/1‑139 (MQ=255)
                                                                                                               cGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg     >  1:242639/1‑139 (MQ=255)
                                                                                                                 aaaGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga   <  2:81203/139‑1 (MQ=255)
                                                                                                                 aaaGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCGCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTATTTTCTCCCTCGCCCCTCCGGGga   <  2:568886/139‑1 (MQ=255)
                                                                                                                  aaGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgag  <  2:242639/139‑1 (MQ=255)
                                                                                                                     ggAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatg                                                             >  1:788307/1‑77 (MQ=255)
                                                                                                                     ggAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatg                                                             <  2:788307/77‑1 (MQ=255)
                                                                                                                        aTGCGGCCTAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                     <  1:560666/82‑1 (MQ=255)
                                                                                                                        aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg               <  1:779478/120‑1 (MQ=255)
                                                                                                                        aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg               >  2:779478/1‑120 (MQ=255)
                                                                                                                        aTGCGGCATAATCGTCTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                     >  2:560666/1‑82 (MQ=255)
                                                                                                                         tGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc                                                                   <  2:186416/67‑1 (MQ=255)
                                                                                                                         tGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc                                                                   >  1:186416/1‑67 (MQ=255)
                                                                                                                                  |                                                                                                                          
AGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAG  >  NC_000913/3780887‑3781139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑