Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,789,047 C→T A316V (GCT→GTT) 
*345* (TAG→TAA
yibQ →
waaH ←
putative polysaccharide deacetylase
LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,789,0470CT100.0% 71.2 / NA 23A316V (GCT→GTT) 
*345* (TAG→TAA
yibQ
waaH
putative polysaccharide deacetylase
LPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/12);  total (11/12)

CTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGG  >  NC_000913/3788924‑3789176
                                                                                                                           |                                                                                                                                 
cTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAg                                                                                                                    >  1:96284/1‑139 (MQ=255)
cTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAg                                                                                                                    <  1:80509/139‑1 (MQ=255)
      aTGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTa                                                                                                              <  1:667872/139‑1 (MQ=255)
           aaTCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcg                                                                                                            >  1:137345/1‑136 (MQ=255)
           aaTCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcg                                                                                                            <  2:137345/136‑1 (MQ=255)
             tCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTa                                                                                                              <  2:722645/132‑1 (MQ=255)
             tCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTa                                                                                                              <  2:403433/132‑1 (MQ=255)
             tCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTa                                                                                                              >  1:722645/1‑132 (MQ=255)
             tCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTa                                                                                                              >  1:403433/1‑132 (MQ=255)
                                            aGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCa                                                                        >  1:319743/1‑139 (MQ=255)
                                               cAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTg                                                                     <  2:319743/139‑1 (MQ=255)
                                                        cTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCg                                                            >  1:541732/1‑139 (MQ=255)
                                                                       cAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTaa                                             >  1:126775/1‑139 (MQ=255)
                                                                        aGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAAc                                            >  1:518037/1‑139 (MQ=255)
                                                                                                 aCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTgc                   >  2:252696/1‑139 (MQ=255)
                                                                                                 aCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTgc                   >  1:120674/1‑139 (MQ=255)
                                                                                                  cAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTgcg                  <  2:126775/139‑1 (MQ=255)
                                                                                                  cAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCCTCACGCTgcg                  <  2:541732/139‑1 (MQ=255)
                                                                                                    gCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGca                <  2:120674/139‑1 (MQ=255)
                                                                                                     cccGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACca                                                   >  1:695848/1‑103 (MQ=255)
                                                                                                     cccGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACca                                                   <  2:695848/103‑1 (MQ=255)
                                                                                                                gAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCa    <  1:665713/139‑1 (MQ=255)
                                                                                                                  aGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAgg  <  2:463906/139‑1 (MQ=255)
                                                                                                                           |                                                                                                                                 
CTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGG  >  NC_000913/3788924‑3789176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: