Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,792,826	C→T	*286* (TAG→TAA)	yibB ←	YibB family protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,792,826	0	C	T	100.0%	54.0 / NA	18	*286* (TAG→TAA)	yibB	YibB family protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

AAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATA  >  NC_000913/3792693‑3792960                                                                                                                                      |                                                                                                                                       aaaCTGTACAGTTTTGACTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTAttt                                                                                                                                   >  2:288944/1‑139 (MQ=255)                                                ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                    >  1:261788/1‑139 (MQ=255)                                                 tGATATAAAAAGAAAAAATGATGCCAAACCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGtt                                                                                   <  1:503029/139‑1 (MQ=255)                                                   atatAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                                                                        >  1:364955/1‑132 (MQ=255)                                                   atatAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                                                                        <  2:364955/132‑1 (MQ=255)                                                                                     tttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTcc                                               <  1:301794/139‑1 (MQ=255)                                                                                        ccTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctct                                            >  1:429957/1‑139 (MQ=255)                                                                                            tctcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTcccc                                        >  2:473314/1‑139 (MQ=255)                                                                                                   tGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                 <  2:429957/139‑1 (MQ=255)                                                                                                   tGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                 <  2:261788/139‑1 (MQ=255)                                                                                                            aaCGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGa                        >  1:610112/1‑139 (MQ=255)                                                                                                                cTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTcc                                          <  1:672213/117‑1 (MQ=255)                                                                                                                cTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTcc                                          >  2:672213/1‑117 (MQ=255)                                                                                                                      aCTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg              <  1:280022/139‑1 (MQ=255)                                                                                                                      aCTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg              <  2:684391/139‑1 (MQ=255)                                                                                                                                ggAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTaa    >  1:174029/1‑139 (MQ=255)                                                                                                                                ggAAACTTATTTACTTGAAGGGAAAATTCTCAGCGACTGCATATTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTGTCCTCTCCCCAGATAGTTTAGTTTGACGAGATCGGGGCGTTTGTaa    <  2:741068/139‑1 (MQ=255)                                                                                                                                  aaaCTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATa  >  1:549859/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       AAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATA  >  NC_000913/3792693‑3792960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑