Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,441 G→A *355* (TAG→TAA
G430G (GGC→GGT
cbrA →
dgoT ←
colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,4410GA100.0% 128.6 / NA 41*355* (TAG→TAA
G430G (GGC→GGT
cbrA
dgoT
colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/21);  total (20/21)

CTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGA  >  NC_000913/3870305‑3870567
                                                                                                                                        |                                                                                                                              
cgtACTGGCGCGCCACCCGCAACCTGCGTTTAACAATCTTCGGCAAGATAGTAAAGAGTCGATGCCTCACCGGACCCTCCTGAAGAAAGTGGACTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGACTAAcc                                                                                                                              >  2:476422/3‑139 (MQ=255)
                        tGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                      <  2:526926/139‑1 (MQ=255)
                          cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                    <  1:105442/139‑1 (MQ=255)
                                              gATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTca                                                                                                                   <  2:529942/104‑1 (MQ=255)
                                              gATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTca                                                                                                                   >  1:529942/1‑104 (MQ=255)
                                                        aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAcg                                                                                                                          >  2:838907/1‑87 (MQ=255)
                                                        aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAcg                                                                                                                          <  1:838907/87‑1 (MQ=255)
                                                         gCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAc                                                                     >  1:688996/1‑139 (MQ=255)
                                                             aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                      >  2:817071/1‑102 (MQ=255)
                                                             aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                      <  1:817071/102‑1 (MQ=255)
                                                              tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                <  2:236149/139‑1 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                    <  1:396317/78‑1 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                    >  2:396317/1‑78 (MQ=255)
                                                                                        gTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCcca                                                                                                          >  2:209068/1‑71 (MQ=255)
                                                                                        gTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCcca                                                                                                          <  1:209068/71‑1 (MQ=255)
                                                                                                     aGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                        >  1:565475/1‑124 (MQ=255)
                                                                                                     aGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                        <  2:565475/124‑1 (MQ=255)
                                                                                                              gCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                    <  1:715181/55‑1 (MQ=255)
                                                                                                              gCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                    >  2:715181/1‑55 (MQ=255)
                                                                                                               cacaTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                      >  1:718528/1‑52 (MQ=255)
                                                                                                               cacaTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                      <  2:718528/52‑1 (MQ=255)
                                                                                                               cacaTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                        <  1:218964/114‑1 (MQ=255)
                                                                                                               cacaTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                        >  2:218964/1‑114 (MQ=255)
                                                                                                                 caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACAt                                                                                                                <  2:287836/40‑1 (MQ=255)
                                                                                                                 caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACAt                                                                                                                >  1:287836/1‑40 (MQ=255)
                                                                                                                 caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                    <  1:556157/52‑1 (MQ=255)
                                                                                                                 caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                    >  2:556157/1‑52 (MQ=255)
                                                                                                                 caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                <  2:60754/88‑1 (MQ=255)
                                                                                                                 caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                >  1:60754/1‑88 (MQ=255)
                                                                                                                 caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                        <  1:406649/112‑1 (MQ=255)
                                                                                                                 caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                        >  2:406649/1‑112 (MQ=255)
                                                                                                                   tatTCCACAGTTGACGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATAAACGCGAcggcgg                                                                >  1:509720/1‑86 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                            >  2:476597/1‑42 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                            <  1:476597/42‑1 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                <  2:509720/86‑1 (MQ=255)
                                                                                                                        cacaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATaa                                                        >  2:510457/1‑89 (MQ=255)
                                                                                                                        cacaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATaa                                                        <  1:510457/89‑1 (MQ=255)
                                                                                                                            gTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGa  >  1:461711/1‑139 (MQ=255)
                                                                                                                               gAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCccacc           >  2:634677/1‑127 (MQ=255)
                                                                                                                               gAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCccacc           <  1:634677/127‑1 (MQ=255)
                                                                                                                                aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                >  1:238356/1‑73 (MQ=255)
                                                                                                                                aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                <  2:238356/73‑1 (MQ=255)
                                                                                                                                        |                                                                                                                              
CTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGA  >  NC_000913/3870305‑3870567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: