Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,077,469	G→A	L7L (TTG→TTA)	yiiD →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,077,469	0	G	A	100.0%	95.2 / NA	31	L7L (TTG→TTA)	yiiD	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/17);  total (14/17)

ACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTGTCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCAAA  >  NC_000913/4077334‑4077591                                                                                                                                        |                                                                                                                           aCTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCa                                                                                                                         <  2:803697/139‑1 (MQ=255) aCTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCa                                                                                                                         <  2:267128/139‑1 (MQ=255)   tATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                       <  1:112585/139‑1 (MQ=255)     tttCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACggg                                                                                                                     >  2:187341/1‑139 (MQ=255)        cgtcgAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGaaa                                                                                                                  >  2:3865/1‑139 (MQ=255)          tcgAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACa                                                                                                                >  2:478597/1‑139 (MQ=255)             aaCGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAaga                                                                                                             <  2:354305/139‑1 (MQ=255)             aaCGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAaga                                                                                                             >  2:719306/1‑139 (MQ=255)                   gCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGt                                                                                                       <  1:658269/139‑1 (MQ=255)                               agaTGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTAt                                                                                           <  1:719306/139‑1 (MQ=255)                                                         gCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaaga                                                                 >  1:752116/1‑139 (MQ=255)                                                                atatGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGGGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTa                                                          >  1:680842/1‑139 (MQ=255)                                                                  atGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTaga                                                        <  1:3865/139‑1 (MQ=255)                                                                          aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAAt                                                            >  1:661448/1‑127 (MQ=255)                                                                          aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAAt                                                            <  2:661448/127‑1 (MQ=255)                                                                                    aaTGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCg                                      <  2:296662/139‑1 (MQ=255)                                                                                         tgGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTggg                                 >  2:432196/1‑139 (MQ=255)                                                                                           gCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGaaa                                                                                                                  <  2:839453/56‑1 (MQ=255)                                                                                           gCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGaaa                                                                                                                  >  1:839453/1‑56 (MQ=255)                                                                                            ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAagagagag                                                                                                        >  2:531653/1‑65 (MQ=255)                                                                                            ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAagagagag                                                                                                        <  1:531653/65‑1 (MQ=255)                                                                                            ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGaaa                              >  2:278502/1‑139 (MQ=255)                                                                                            ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGaaa                              >  2:52197/1‑139 (MQ=255)                                                                                              ccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATg                            <  1:432196/139‑1 (MQ=255)                                                                                              ccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATg                            <  1:187341/139‑1 (MQ=255)                                                                                                                aGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCAt          <  2:680842/139‑1 (MQ=255)                                                                                                                 ggTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTa                     <  2:484947/127‑1 (MQ=255)                                                                                                                 ggTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTa                     >  1:484947/1‑127 (MQ=255)                                                                                                                     tGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCg                                                     >  1:838134/1‑91 (MQ=255)                                                                                                                     tGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCg                                                     <  2:838134/91‑1 (MQ=255)                                                                                                                        gCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCaaa  <  1:630485/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                           ACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTGTCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCAAA  >  NC_000913/4077334‑4077591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑