Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,148,681 C→T G529S (GGC→AGC)  eptC ← LPS heptose I phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,148,6810CT100.0% 134.8 / NA 43G529S (GGC→AGC) eptCLPS heptose I phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/21);  total (22/21)

ATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGT  >  NC_000913/4148556‑4148806
                                                                                                                             |                                                                                                                             
aTAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                  <  2:391453/139‑1 (MQ=255)
aTAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                  <  1:190478/139‑1 (MQ=255)
 tAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                 <  1:381781/139‑1 (MQ=255)
         tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCGGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                         >  1:410508/1‑139 (MQ=255)
         tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                         >  1:62823/1‑139 (MQ=255)
         tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                         >  1:530022/1‑139 (MQ=255)
                       tCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAg                                                                                           >  2:237722/1‑139 (MQ=255)
                       tCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAg                                                                                           >  1:23529/1‑139 (MQ=255)
                        cAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                          >  2:416474/1‑139 (MQ=255)
                         aGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                         <  2:820022/139‑1 (MQ=255)
                           tGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCATTTCCGCCAGGCTg                                                                                       >  1:110440/1‑139 (MQ=255)
                                             gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGcc                                                                                             <  1:309081/115‑1 (MQ=255)
                                             gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGcc                                                                                             >  2:309081/1‑115 (MQ=255)
                                                   aaTCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGACGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCgag                                                               <  2:530022/139‑1 (MQ=255)
                                                     tCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                  <  2:174264/86‑1 (MQ=255)
                                                     tCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                  >  1:174264/1‑86 (MQ=255)
                                                       cAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAAt                                                           >  1:122253/1‑139 (MQ=255)
                                                        aGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATc                                                          >  1:201315/1‑139 (MQ=255)
                                                          cGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCAc                                                        >  2:313831/1‑139 (MQ=255)
                                                                gTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGAt                                                  <  1:188604/139‑1 (MQ=255)
                                                                              cGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGAt                                                                                                       >  1:674830/1‑72 (MQ=255)
                                                                              cGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGAt                                                                                                       <  2:674830/72‑1 (MQ=255)
                                                                              cGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCAt                                    >  1:824801/1‑139 (MQ=255)
                                                                                     accacTGACCCGGGTGGATCGTATCCGTCGTAAGATAAGGTCGCCAAGTCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATGAACATCCTGTGAGAAATCCCGGGGATGAGTGGCATGCCATTTTTCTg                             <  2:410508/139‑1 (MQ=255)
                                                                                       cacTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGAc                           <  2:62823/139‑1 (MQ=255)
                                                                                        acTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACCATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGAcc                          >  1:93625/1‑138 (MQ=255)
                                                                                          tGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTc                        >  2:783733/1‑139 (MQ=255)
                                                                                            aaCGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                 >  1:68842/1‑48 (MQ=255)
                                                                                            aaCGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                 <  2:68842/48‑1 (MQ=255)
                                                                                                 gTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                  >  1:620014/1‑42 (MQ=255)
                                                                                                 gTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                  <  2:620014/42‑1 (MQ=255)
                                                                                                 gTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGa                                                                                                        >  2:176798/1‑52 (MQ=255)
                                                                                                 gTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGa                                                                                                        <  1:176798/52‑1 (MQ=255)
                                                                                                         gTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa         <  2:824801/139‑1 (MQ=255)
                                                                                                         gTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa         <  1:675004/139‑1 (MQ=255)
                                                                                                          tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                          >  2:219838/1‑57 (MQ=255)
                                                                                                          tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                          <  1:219838/57‑1 (MQ=255)
                                                                                                          tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGTCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa        <  2:122253/139‑1 (MQ=255)
                                                                                                          tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa        <  2:98453/139‑1 (MQ=255)
                                                                                                          tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa        <  1:783733/139‑1 (MQ=255)
                                                                                                               gtcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGAAACGGAATGgtg   <  1:122355/139‑1 (MQ=255)
                                                                                                                tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACgg          >  1:402868/1‑131 (MQ=255)
                                                                                                                tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACgg          <  2:402868/131‑1 (MQ=255)
                                                                                                                tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGgtgt  >  2:552521/1‑139 (MQ=255)
                                                                                                                             |                                                                                                                             
ATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGT  >  NC_000913/4148556‑4148806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑