Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,232,752	G→A	A161V (GCG→GTG)	lysC ←	lysine‑sensitive aspartokinase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,232,752	0	G	A	100.0%	144.5 / NA	45	A161V (GCG→GTG)	lysC	lysine‑sensitive aspartokinase 3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/24);  total (21/24)

GCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCGCGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGC  >  NC_000913/4232634‑4232881                                                                                                                       |                                                                                                                                  gCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCa                                                                                                               >  1:831418/1‑139 (MQ=255)              aGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGt                                                                                                  >  1:401456/1‑139 (MQ=255)                cgtcgtTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCg                                                                                                <  2:401456/139‑1 (MQ=255)                         aCGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGc                                                                                       <  2:831418/139‑1 (MQ=255)                                  aTTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCt                                                                                                                              >  2:141263/1‑91 (MQ=255)                                  aTTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCt                                                                                                                              <  1:141263/91‑1 (MQ=255)                                    tttCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                            <  1:511910/139‑1 (MQ=255)                                         cTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCa                                                                                                               <  2:677174/99‑1 (MQ=255)                                         cTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCa                                                                                                               >  1:677174/1‑99 (MQ=255)                                               aTAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGt                                                                                           <  1:333917/113‑1 (MQ=255)                                               aTAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGt                                                                                           >  2:333917/1‑113 (MQ=255)                                                      ccTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTgg                                                                                                                      >  2:41965/1‑79 (MQ=255)                                                      ccTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTgg                                                                                                                      <  1:41965/79‑1 (MQ=255)                                                               tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                                      >  1:396692/1‑102 (MQ=255)                                                               tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                                      <  2:396692/102‑1 (MQ=255)                                                                        cTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATc                                                                                                                         >  1:27998/1‑58 (MQ=255)                                                                        cTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATc                                                                                                                         <  2:27998/58‑1 (MQ=255)                                                                        cTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCAt                                                                                     >  2:791971/1‑94 (MQ=255)                                                                        cTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCAt                                                                                     <  1:791971/94‑1 (MQ=255)                                                                         ttCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCACATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCgcgc                                       <  2:572351/139‑1 (MQ=255)                                                                          tCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCa                                      >  1:403386/1‑139 (MQ=255)                                                                           cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                            <  1:701798/100‑1 (MQ=255)                                                                           cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                            >  2:701798/1‑100 (MQ=255)                                                                            aTTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAgg                                    >  1:163683/1‑139 (MQ=255)                                                                             ttGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGt                                                                                           >  1:368942/1‑83 (MQ=255)                                                                             ttGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGt                                                                                           <  2:368942/83‑1 (MQ=255)                                                                             ttGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGa                                   >  2:216770/1‑139 (MQ=255)                                                                             ttGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGa                                   <  1:216770/139‑1 (MQ=255)                                                                                agaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGAtct                                <  2:163683/139‑1 (MQ=255)                                                                                 gaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTg                                                                                             <  1:515703/77‑1 (MQ=255)                                                                                 gaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTg                                                                                             >  2:515703/1‑77 (MQ=255)                                                                                    gTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGAtct                                >  2:307470/1‑135 (MQ=255)                                                                                    gTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGAtct                                <  1:307470/135‑1 (MQ=255)                                                                                       ggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc                                                               <  2:582421/101‑1 (MQ=255)                                                                                       ggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTAAGCATCACTTTACGTACATCAAACCAc                                                               >  1:582421/1‑101 (MQ=255)                                                                                                 cAGCGCGGCCAGTTCCGCCAGCACGGATATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTc               <  2:114944/139‑1 (MQ=255)                                                                                                      cgGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCaaa                                                                                                       >  2:151504/1‑46 (MQ=255)                                                                                                      cgGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCaaa                                                                                                       <  1:151504/46‑1 (MQ=255)                                                                                                      cgGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                                      <  1:408535/63‑1 (MQ=255)                                                                                                      cgGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                                      >  2:408535/1‑63 (MQ=255)                                                                                                        gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                            >  2:742434/1‑71 (MQ=255)                                                                                                        gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                            <  1:742434/71‑1 (MQ=255)                                                                                                        gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCa        >  1:168788/1‑139 (MQ=255)                                                                                                         ccAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAg       <  2:168788/139‑1 (MQ=255)                                                                                                              tCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGc  <  1:556092/139‑1 (MQ=255)                                                                                                                       |                                                                                                                                  GCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCGCGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGC  >  NC_000913/4232634‑4232881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑