Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,425,834 G→A *105* (TAG→TAA priB → primosomal protein N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,425,8340GA100.0% 87.7 / NA 28*105* (TAG→TAApriBprimosomal protein N
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

TTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAG  >  NC_000913/4425704‑4425963
                                                                                                                                  |                                                                                                                                 
ttACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATgg                                                                                                                           <  2:744627/139‑1 (MQ=255)
ttACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATgg                                                                                                                           <  2:593130/139‑1 (MQ=255)
            taACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTcc                                                                                                               >  1:525775/1‑139 (MQ=255)
                    ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGc                                                                                                       >  1:156007/1‑139 (MQ=255)
                          cGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTc                                                                                                 <  2:424651/139‑1 (MQ=255)
                                    ttCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCa                                                                                       >  2:649750/1‑139 (MQ=255)
                                             tcatttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                              <  2:156007/139‑1 (MQ=255)
                                                           aaGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                <  1:29890/139‑1 (MQ=255)
                                                           aaGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                <  2:525775/139‑1 (MQ=255)
                                                             ggCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                              >  2:90231/1‑139 (MQ=255)
                                                                  aGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACtata                                                         >  2:672415/1‑139 (MQ=255)
                                                                                        tttGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaaaa                                   <  1:90231/139‑1 (MQ=255)
                                                                                            cATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                <  1:810203/106‑1 (MQ=255)
                                                                                            cATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                >  2:810203/1‑106 (MQ=255)
                                                                                                   aGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcg                                                                                                              <  1:818889/53‑1 (MQ=255)
                                                                                                   aGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcg                                                                                                              >  2:818889/1‑53 (MQ=255)
                                                                                                        attgaattgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                              >  2:58593/1‑80 (MQ=255)
                                                                                                        attgaattgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                              <  1:58593/80‑1 (MQ=255)
                                                                                                               tgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                                 >  1:653125/1‑86 (MQ=255)
                                                                                                               tgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                                 <  2:653125/86‑1 (MQ=255)
                                                                                                                  tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTg         >  2:657964/1‑139 (MQ=255)
                                                                                                                      ttCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaa                      >  2:835651/1‑122 (MQ=255)
                                                                                                                      ttCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaa                      <  1:835651/122‑1 (MQ=255)
                                                                                                                       tCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGa                                       <  2:406319/104‑1 (MQ=255)
                                                                                                                       tCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGa                                       >  1:406319/1‑104 (MQ=255)
                                                                                                                         tGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAg  >  2:437206/1‑139 (MQ=255)
                                                                                                                           gagaCTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                <  2:347214/75‑1 (MQ=255)
                                                                                                                           gagaCTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                >  1:347214/1‑75 (MQ=255)
                                                                                                                                  |                                                                                                                                 
TTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAG  >  NC_000913/4425704‑4425963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: