Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,441,175 A→G V14A (GTA→GCA)  ytfL ← UPF0053 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,441,1750AG100.0% 110.8 / NA 32V14A (GTA→GCA) ytfLUPF0053 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/17);  total (15/17)

AAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAG  >  NC_000913/4441043‑4441299
                                                                                                                                    |                                                                                                                            
aaGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGa                                                                                                                        <  2:600896/139‑1 (MQ=255)
  gAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATc                                                                                                                      <  2:760015/139‑1 (MQ=255)
      aTGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGc                                                                                                                  >  2:198396/1‑139 (MQ=255)
             gATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTAt                                                                                                           <  1:198396/139‑1 (MQ=255)
                            tCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATTGAGAAGAATGCACTTGCAGCGATCAAGCAGAGt                                                                                                             <  2:387583/122‑1 (MQ=255)
                            tCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATTGAGAAGAATGCACTTGCAGCGATCAAGCAGAGt                                                                                                             >  1:387583/1‑122 (MQ=255)
                                 aCGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACa                                                                                       >  1:529866/1‑139 (MQ=255)
                                   gcgTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCAGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACata                                                                                     <  2:472780/139‑1 (MQ=255)
                                     gTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATc                                                                                   >  2:541470/1‑139 (MQ=255)
                                            tataaatgcgCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                   <  2:708978/91‑1 (MQ=255)
                                            tataaatgcgCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                   >  1:708978/10‑100 (MQ=255)
                                             tttATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGa                                                                           <  1:705630/139‑1 (MQ=255)
                                                   ttGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTc                                                                     >  1:807714/1‑139 (MQ=255)
                                                      ccTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTAt                                                                                                           >  1:550910/1‑98 (MQ=255)
                                                      ccTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTAt                                                                                                           <  2:550910/98‑1 (MQ=255)
                                                        ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                   >  1:494442/1‑88 (MQ=255)
                                                        ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                   <  2:494442/88‑1 (MQ=255)
                                                        ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTg                                                                                              >  2:377865/1‑109 (MQ=255)
                                                        ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTg                                                                                              <  1:377865/109‑1 (MQ=255)
                                                          catcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATa                                                                                                 <  2:114124/104‑1 (MQ=255)
                                                          catcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATa                                                                                                 >  1:114124/1‑104 (MQ=255)
                                                                ccagcAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                   >  1:387417/1‑80 (MQ=255)
                                                                ccagcAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                   <  2:387417/80‑1 (MQ=255)
                                                                       tttAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGAc                                                                                                        <  2:439133/84‑1 (MQ=255)
                                                                       tttAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGAc                                                                                                        >  1:439133/1‑84 (MQ=255)
                                                                               tGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGt                                                                                                             >  2:428602/1‑71 (MQ=255)
                                                                               tGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGt                                                                                                             <  1:428602/71‑1 (MQ=255)
                                                                                     tGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                   >  2:52573/1‑59 (MQ=255)
                                                                                     tGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                   <  1:52573/59‑1 (MQ=255)
                                                                                          gAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTtgtgt                              <  1:278291/139‑1 (MQ=255)
                                                                                              cggcAAGCGAGATCGCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTCAAATACTGTTTAACATATCTTATCGGACCTTTCAGGCCAGATCCTCGGAAGCGGAAGTTGATTAATTTATGTGaaa                          <  1:512970/139‑1 (MQ=255)
                                                                                                                      agaagaACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCgaag  >  1:26900/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                            
AAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAG  >  NC_000913/4441043‑4441299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: