Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,490,141 C→T *333* (TAG→TAA idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,490,1410CT100.0% 115.7 / NA 37*333* (TAG→TAAidnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/19);  total (18/19)

GGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGG  >  NC_000913/4490040‑4490260
                                                                                                     |                                                                                                                       
tccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGt                                                                                    <  2:75914‑M2/52‑1 (MQ=255)
                          ttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                         >  1:192546‑M2/62‑108 (MQ=255)
                          ttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                         <  2:192546‑M2/47‑1 (MQ=255)
                           tcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCgtg                                                                                                                <  2:628604‑M2/24‑1 (MQ=255)
                           tcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCgtg                                                                                                                >  1:628604‑M2/61‑84 (MQ=255)
                             actcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCgtg                                                                                                                <  1:120881‑M2/24‑1 (MQ=255)
                             actcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCgtg                                                                                                                >  2:120881‑M2/59‑82 (MQ=255)
                                       tatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                      >  2:717265‑M2/49‑98 (MQ=255)
                                       tatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                      <  1:717265‑M2/50‑1 (MQ=255)
                                        atcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCt                                            >  2:748793‑M2/48‑139 (MQ=255)
                                         tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGAt                                                                                                      >  2:820647‑M2/47‑80 (MQ=255)
                                         tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGAt                                                                                                      <  1:820647‑M2/34‑1 (MQ=255)
                                         tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAAtt                                                 >  1:320284‑M2/47‑133 (MQ=255)
                                         tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAAtt                                                 <  2:320284‑M2/87‑1 (MQ=255)
                                           ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAg                                         >  1:668520‑M2/45‑139 (MQ=255)
                                               agtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAAc                                     <  1:748793‑M2/99‑1 (MQ=255)
                                                     ctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                            >  1:708271‑M2/35‑62 (MQ=255)
                                                     ctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                            <  2:708271‑M2/28‑1 (MQ=255)
                                                     ctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTgg                               >  2:181311‑M2/35‑139 (MQ=255)
                                                        cctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGggcg                            <  1:265507‑M2/108‑1 (MQ=255)
                                                                  aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGGGTTGCCATGATAAATCTGATACCCGAGGTCGAGTGTGGTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGAGGCCATCCGAc                  >  2:65127‑M2/22‑139 (MQ=255)
                                                                  aggcaacgtccggcaaagagaGAAGAGAAAATAACTGAAAGCGTGGTGCCGTGATAAATCTGATACTCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGAc                  <  2:154617‑M2/118‑1 (MQ=255)
                                                                       acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTgttgtggttgt                                                                  <  2:413925‑M2/70‑1 (MQ=255)
                                                                       acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTgttgtggttgt                                                                  >  1:413925‑M2/17‑86 (MQ=255)
                                                                        cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAc                                                                                           >  2:518527‑M2/16‑60 (MQ=255)
                                                                        cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAc                                                                                           <  1:518527‑M2/45‑1 (MQ=255)
                                                                        cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGATGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCt                                            >  2:174377‑M2/16‑107 (MQ=255)
                                                                        cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGATGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCt                                            <  1:174377‑M2/92‑1 (MQ=255)
                                                                        cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAatat            >  2:447246‑M2/16‑139 (MQ=255)
                                                                             ggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                 >  1:781412‑M2/11‑65 (MQ=255)
                                                                             ggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                 <  2:781412‑M2/55‑1 (MQ=255)
                                                                              gcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAAc      <  1:447246‑M2/130‑1 (MQ=255)
                                                                                  agagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACgggg  <  1:671969‑M2/134‑1 (MQ=255)
                                                                                              aaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttg                                                                         <  2:454083/56‑1 (MQ=255)
                                                                                              aaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttg                                                                         >  1:454083/1‑56 (MQ=255)
                                                                                              aaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGc                                        <  2:664928/89‑1 (MQ=255)
                                                                                              aaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGc                                        >  1:664928/1‑89 (MQ=255)
                                                                                                     |                                                                                                                       
GGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGG  >  NC_000913/4490040‑4490260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: