Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,690	C→T	*256* (TAG→TAA)	fecE ←	ferric citrate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,690	0	C	T	100.0%	105.0 / NA	33	*256* (TAG→TAA)	fecE	ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/17);  total (16/17)

GCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATCTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGTTT  >  NC_000913/4510565‑4510822                                                                                                                              |                                                                                                                                     gcGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGc                                                                                                                         >  2:212995/1‑139 (MQ=255)                      gTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCg                                                                                                    >  1:709590/1‑139 (MQ=255)                         aTCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                                 >  1:2599/1‑139 (MQ=255)                         aTCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                                 >  2:439275/1‑139 (MQ=255)                           cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGcc                                                                                                                <  2:800426/122‑1 (MQ=255)                           cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGcc                                                                                                                >  1:800426/1‑122 (MQ=255)                           cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                               <  2:2599/139‑1 (MQ=255)                           cTTTCAGAGTCCAGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                               <  2:78080/139‑1 (MQ=255)                           cTTTCAGAGTCCAGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                               >  1:78080/1‑139 (MQ=255)                                    tCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTAtt                                                                                      >  1:340046/1‑139 (MQ=255)                                      cGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTc                                                                                    >  1:496677/1‑139 (MQ=255)                                             gTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTcc                                                                             <  2:709590/139‑1 (MQ=255)                                              ttCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCa                                                                            >  2:360382/1‑139 (MQ=255)                                               tCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCAc                                                                           <  1:439275/139‑1 (MQ=255)                                                cAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACg                                                                          <  2:424160/139‑1 (MQ=255)                                                                 ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                         <  2:455009/139‑1 (MQ=255)                                                                                 tccagtTTCCTTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGtcatc                                         <  1:775615/139‑1 (MQ=255)                                                                                 tccagtTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                       >  1:90945/1‑77 (MQ=255)                                                                                 tccagtTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                       <  2:90945/77‑1 (MQ=255)                                                                                       ttcattcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACctct                                   <  1:131695/139‑1 (MQ=255)                                                                                                   tggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACg                                                                          <  2:397643/88‑1 (MQ=255)                                                                                                   tggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACg                                                                          >  1:397643/1‑88 (MQ=255)                                                                                                    ggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCtt                      <  1:360382/139‑1 (MQ=255)                                                                                                     gtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTg                     <  2:555302/139‑1 (MQ=255)                                                                                                          gttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCa                <  1:94297/139‑1 (MQ=255)                                                                                                          gttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCa                >  2:546598/1‑139 (MQ=255)                                                                                                                  ggCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCt                       <  2:735728/124‑1 (MQ=255)                                                                                                                  ggCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCt                       >  1:735728/1‑124 (MQ=255)                                                                                                                        tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGtc                                            >  2:25980/1‑97 (MQ=255)                                                                                                                        tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGtc                                            <  1:25980/97‑1 (MQ=255)                                                                                                                        tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGttt  >  1:629204/1‑139 (MQ=255)                                                                                                                        tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGt    <  1:752780/137‑1 (MQ=255)                                                                                                                        tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGt    >  2:752780/1‑137 (MQ=255)                                                                                                                              |                                                                                                                                     GCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATCTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCGTTT  >  NC_000913/4510565‑4510822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑