Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,538,785	C→T	*239* (TAG→TAA)	nanC ←	N‑acetylnuraminic acid outer membrane channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,538,785	0	C	T	100.0%	147.3 / NA	46	*239* (TAG→TAA)	nanC	N‑acetylnuraminic acid outer membrane channel protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/25);  total (21/25)

TGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTACTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATT  >  NC_000913/4538682‑4538912                                                                                                        |                                                                                                                                tGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGtt                                                                                              <  1:400390/139‑1 (MQ=255)              tCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTa                                                                                 >  2:390480/1‑139 (MQ=255)                                    cGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCt                                                           <  1:56170/139‑1 (MQ=255)                                               gCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                <  1:447740/139‑1 (MQ=255)                                                   tcatGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGt                                            <  1:390480/139‑1 (MQ=255)                                                    catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGt                                            >  1:52853/1‑138 (MQ=255)                                                    catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGt                                            <  2:52853/138‑1 (MQ=255)                                                    catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                           <  1:105169/139‑1 (MQ=255)                                                    catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                           <  2:774679/139‑1 (MQ=255)                                                    catGATAGCAAGCGCCGTTATTGTTCTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                           <  2:460127/139‑1 (MQ=255)                                                     atGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCa                                          <  2:196842/139‑1 (MQ=255)                                                      tGATAGCAAGCGCCGGTATTGGTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCat                                         <  1:444358/139‑1 (MQ=255)                                                              aGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGAc                                                        >  1:354246/1‑116 (MQ=255)                                                              aGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGAc                                                        <  2:354246/116‑1 (MQ=255)                                                               gcgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCat                                         <  1:288811/130‑1 (MQ=255)                                                               gcgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCat                                         >  2:288811/1‑130 (MQ=255)                                                                    gTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATaaa                                                                                    >  1:722027/1‑82 (MQ=255)                                                                    gTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATaaa                                                                                    <  2:722027/82‑1 (MQ=255)                                                                    gTTATTGTTTTATTCATTGAGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGc                           >  1:558790/1‑139 (MQ=255)                                                                     ttATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                >  2:605704/1‑117 (MQ=255)                                                                     ttATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                <  1:605704/117‑1 (MQ=255)                                                                     ttATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCg                          >  1:471912/1‑139 (MQ=255)                                                                             ttATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGa                                                                                        >  2:577223/1‑69 (MQ=255)                                                                             ttATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGa                                                                                        <  1:577223/69‑1 (MQ=255)                                                                               aTTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                           <  2:779384/80‑1 (MQ=255)                                                                               aTTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                           >  1:779384/1‑80 (MQ=255)                                                                                ttCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                <  2:623118/106‑1 (MQ=255)                                                                                ttCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                >  1:623118/1‑106 (MQ=255)                                                                                 tCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCg              <  1:302690/139‑1 (MQ=255)                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGa                                                                                        >  2:288723/1‑61 (MQ=255)                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGa                                                                                        <  1:288723/61‑1 (MQ=255)                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGt                                                     >  2:229171/1‑96 (MQ=255)                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGt                                                     <  1:229171/96‑1 (MQ=255)                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                           <  1:575473/106‑1 (MQ=255)                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                           >  2:575473/1‑106 (MQ=255)                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCat          >  1:483487/1‑139 (MQ=255)                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTCCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCat          >  1:122598/1‑139 (MQ=255)                                                                                          cTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCggg                >  2:677031/1‑128 (MQ=255)                                                                                          cTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCggg                <  1:677031/128‑1 (MQ=255)                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTAtt  >  1:369968/1‑139 (MQ=255)                                                                                              ctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGa                                                         >  2:579099/1‑83 (MQ=255)                                                                                              ctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGa                                                         <  1:579099/83‑1 (MQ=255)                                                                                               tcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                          >  1:137933/1‑81 (MQ=255)                                                                                               tcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                          <  2:137933/81‑1 (MQ=255)                                                                                                    tCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCg              >  2:578698/1‑120 (MQ=255)                                                                                                    tCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCg              <  1:578698/120‑1 (MQ=255)                                                                                                        |                                                                                                                                TGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTACTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATT  >  NC_000913/4538682‑4538912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑