Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,567,249	C→T	intergenic (‑3/+38)	yjiN ← / ← mdtM	zinc‑type alcohol dehydrogenase‑like protein/multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,567,249	0	C	T	100.0%	35.0 / NA	13	intergenic (‑3/+38)	yjiN/mdtM	zinc‑type alcohol dehydrogenase‑like protein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)

AGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACG  >  NC_000913/4567115‑4567368                                                                                                                                       |                                                                                                                        aGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAATGTTa                                                                                                                     >  2:30764/1‑139 (MQ=255)       cGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAATGTTAAGAATa                                                                                                               <  2:741939/139‑1 (MQ=255)                    cTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAATGTTAAGAATAGAGCCTGACGGcc                                                                                                  >  2:194125/1‑139 (MQ=255)                                      ggcAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAATGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCAc                                                                                <  1:194125/139‑1 (MQ=255)                                                         cAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAATGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCgg                                                             <  2:562162/139‑1 (MQ=255)                                                                                     agagagCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAATGtt                                                                                                                      <  1:232201/54‑1 (MQ=255)                                                                                     agagagCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAATGtt                                                                                                                      >  2:232201/1‑54 (MQ=255)                                                                                         agCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAATGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAAt                             >  1:621027/1‑139 (MQ=255)                                                                                          gcgcCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAATGTTAAGAATAGAGCCt                                                                                                         >  1:704767/1‑62 (MQ=255)                                                                                          gcgcCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAATGTTAAGAATAGAGCCt                                                                                                         <  2:704767/62‑1 (MQ=255)                                                                                                        gcgcGTCTGAGTTCGATGAGTTTATTCATAATGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCgg                                                             >  1:357308/1‑92 (MQ=255)                                                                                                        gcgcGTCTGAGTTCGATGAGTTTATTCATAATGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCgg                                                             <  2:357308/92‑1 (MQ=255)                                                                                                                    tCGATGAGTTTATTCATAATGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAAcg  <  1:30764/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                        AGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACG  >  NC_000913/4567115‑4567368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑