Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,569,309 G→A pseudogene (312/921 nt) yjiP → pseudogene, transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,569,3090GA100.0% 124.1 / NA 39pseudogene (312/921 nt)yjiPpseudogene, transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/16);  total (23/16)

CGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGAC  >  NC_000913/4569173‑4569431
                                                                                                                                        |                                                                                                                          
cgcTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                          <  2:526534/139‑1 (MQ=255)
         ccGATATTTCATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATaga                                                                                                                 <  2:494777/139‑1 (MQ=255)
          cGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTACATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATagag                                                                                                                <  1:332291/139‑1 (MQ=255)
                      gTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGc                                                                                                    >  1:179886/1‑139 (MQ=255)
                         ggTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAg                                                                                                 >  1:606966/1‑139 (MQ=255)
                            aaaGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCg                                                                                              <  2:179886/139‑1 (MQ=255)
                                     cgAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTg                                                                                     >  1:216507/1‑139 (MQ=255)
                                     cgAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTg                                                                                     >  1:220260/1‑139 (MQ=255)
                                     cgAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTg                                                                                     >  1:32688/1‑139 (MQ=255)
                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                >  2:478221/1‑139 (MQ=255)
                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                >  1:377160/1‑139 (MQ=255)
                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                >  2:327769/1‑139 (MQ=255)
                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                >  1:213713/1‑139 (MQ=255)
                                                              gtgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACg                                                            >  1:793704/1‑139 (MQ=255)
                                                               tgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                           <  2:377160/139‑1 (MQ=255)
                                                               tgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                           >  1:620740/1‑139 (MQ=255)
                                                                gATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          <  2:620740/139‑1 (MQ=255)
                                                                 aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGAt                                                                          <  2:256691/122‑1 (MQ=255)
                                                                 aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGAt                                                                          >  1:256691/1‑122 (MQ=255)
                                                                    gAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                           >  2:105470/1‑134 (MQ=255)
                                                                    gAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                           <  1:105470/134‑1 (MQ=255)
                                                                            gagCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCt                                              <  2:793704/139‑1 (MQ=255)
                                                                             agCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCtt                                             >  2:817004/1‑139 (MQ=255)
                                                                                cgcgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          >  2:28442/1‑123 (MQ=255)
                                                                                cgcgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          <  1:28442/123‑1 (MQ=255)
                                                                                  cgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTAcccc                                        >  2:525309/1‑139 (MQ=255)
                                                                                   gAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCt                                       <  1:478221/139‑1 (MQ=255)
                                                                                    aGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTg                                      >  2:1707/1‑139 (MQ=255)
                                                                                    aGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTg                                      >  1:260090/1‑139 (MQ=255)
                                                                                             atatGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCtgtg                             <  1:525309/139‑1 (MQ=255)
                                                                                                  gCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATg                                                                         >  1:147932/1‑90 (MQ=255)
                                                                                                  gCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTgctggc                        <  1:817004/139‑1 (MQ=255)
                                                                                                  gCCTTTCCCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATg                                                                         <  2:147932/90‑1 (MQ=255)
                                                                                                   ccTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                          <  2:571249/40‑1 (MQ=255)
                                                                                                   ccTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                          >  1:571249/1‑40 (MQ=255)
                                                                                                   ccTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          >  1:643205/1‑104 (MQ=255)
                                                                                                   ccTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          <  2:643205/104‑1 (MQ=255)
                                                                                                    cTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTgctggctg                      >  2:416261/1‑139 (MQ=255)
                                                                                                     tttCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          >  2:202811/1‑102 (MQ=255)
                                                                                                     tttCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          <  1:202811/102‑1 (MQ=255)
                                                                                                       tCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                          >  1:49551/1‑36 (MQ=255)
                                                                                                                   cTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGccgac       >  1:95013/1‑139 (MQ=255)
                                                                                                                        ccATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGccgacccgac  <  2:260090/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                          
CGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGAC  >  NC_000913/4569173‑4569431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: