Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	491,776	T→C	V122A (GTT→GCT)	apt →	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	491,776	0	T	C	100.0%	93.6 / NA	27	V122A (GTT→GCT)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/14);  total (13/14)

GTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGAT  >  NC_000913/491664‑491891                                                                                                                 |                                                                                                                    gTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCaa                                                                                           >  2:674622/1‑139 (MQ=255) gTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCaa                                                                                           >  1:520798/1‑139 (MQ=255)             ggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGTGGCa                                                                                  <  2:640940/136‑1 (MQ=255)             ggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCa                                                                                  >  1:632178/1‑136 (MQ=255)             ggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCa                                                                                  >  1:640940/1‑136 (MQ=255)             ggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCa                                                                                  <  2:632178/136‑1 (MQ=255)                    tGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc                                                                        <  2:520798/139‑1 (MQ=255)                    tGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc                                                                        <  1:674622/139‑1 (MQ=255)                         cgTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCg                                                                   <  2:722932/139‑1 (MQ=255)                                          aaCTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgt                                                     <  1:158962/136‑1 (MQ=255)                                          aaCTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgt                                                     >  2:158962/1‑136 (MQ=255)                                               aCGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                                    <  1:466687/100‑1 (MQ=255)                                               aCGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                                    >  2:466687/1‑100 (MQ=255)                                                                  cGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCt                          >  2:695897/1‑139 (MQ=255)                                                                    aTCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcg                                                                                        <  1:262364/75‑1 (MQ=255)                                                                    aTCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcg                                                                                        >  2:262364/1‑75 (MQ=255)                                                                       aGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                     <  1:695897/139‑1 (MQ=255)                                                                       aGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                     <  2:76666/139‑1 (MQ=255)                                                                              gaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTg                         <  1:678145/128‑1 (MQ=255)                                                                              gaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTg                         >  2:678145/1‑128 (MQ=255)                                                                                       tGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCg                                                                   <  2:638157/77‑1 (MQ=255)                                                                                       tGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCg                                                                   >  1:638157/1‑77 (MQ=255)                                                                                          tGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGAt  >  1:168904/1‑139 (MQ=255)                                                                                           gCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCg                                                                   <  1:811929/73‑1 (MQ=255)                                                                                           gCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCg                                                                   >  2:811929/1‑73 (MQ=255)                                                                                                   aCCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATc                                                        >  2:334423/1‑76 (MQ=255)                                                                                                   aCCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATc                                                        <  1:334423/76‑1 (MQ=255)                                                                                                                 |                                                                                                                    GTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGAT  >  NC_000913/491664‑491891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑