Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,006,415	Δ1 bp	coding (464/543 nt)	zapC →	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,006,413	0	G	.	100.0%	141.6 / NA	34	coding (462/543 nt)	zapC	FtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (17/17);  total (17/17)

CTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAA  >  NC_000913/1006290‑1006537                                                                                                                            |                                                                                                                             cTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaaa                                                                                                              <  1:292942/139‑1 (MQ=255)         ggTTGAGCGATACTCACGCGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcga                                                                                                                          >  1:130908/1‑117 (MQ=255)         ggTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcga                                                                                                                          <  2:130908/120‑4 (MQ=255)              aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGTGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGc                                                                                                                      <  2:557362/118‑1 (MQ=255)              aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGTGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGc                                                                                                                      >  1:557362/1‑118 (MQ=255)              aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAg                                                                                                 >  1:450816/1‑139 (MQ=255)              aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAg                                                                                                 >  1:507997/1‑139 (MQ=255)                   tACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGa                                                                                            >  2:65910/1‑139 (MQ=255)                       cACGACCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAAcc                                                                                        <  1:976948/139‑1 (MQ=255)                             cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAgg                                                                                   >  1:965975/1‑138 (MQ=255)                             cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAgg                                                                                   <  2:965975/138‑1 (MQ=255)                               ggTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATg                                                                                                         >  1:129747/1‑114 (MQ=255)                               ggTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATg                                                                                                         <  2:129747/114‑1 (MQ=255)                               ggTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                >  2:685713/1‑139 (MQ=255)                               ggTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                <  1:488694/139‑1 (MQ=255)                                            gTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGaagctgt                                                                   <  1:891826/139‑7 (MQ=255)                                                                        tATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGcc                                       <  2:989774/139‑1 (MQ=255)                                                                        tATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGcc                                       <  1:685713/139‑1 (MQ=255)                                                                                    cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                    >  1:661613/1‑66 (MQ=255)                                                                                    cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                    <  2:661613/66‑1 (MQ=255)                                                                                      caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                         <  2:264208/139‑1 (MQ=255)                                                                                             gCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGACTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                         >  2:134617/1‑132 (MQ=255)                                                                                             gCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGACTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                         <  1:134617/132‑1 (MQ=255)                                                                                              cGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                      <  2:326372/134‑1 (MQ=255)                                                                                              cGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                      >  1:326372/1‑134 (MQ=255)                                                                                                   ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                <  1:95062/71‑1 (MQ=255)                                                                                                   ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                >  2:95062/1‑71 (MQ=255)                                                                                                   ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTa                 <  2:504039/134‑1 (MQ=255)                                                                                                   ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTa                 >  1:504039/1‑134 (MQ=255)                                                                                                   ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg            >  1:469812/1‑139 (MQ=255)                                                                                                           ggTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCCGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTATACAGCTACAGCa    >  1:775639/1‑139 (MQ=255)                                                                                                             tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                             <  2:383858/112‑1 (MQ=255)                                                                                                             tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                             >  1:383858/1‑112 (MQ=255)                                                                                                             tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa  >  2:601204/1‑139 (MQ=255)                                                                                                                     tGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                >  1:535983/1‑53 (MQ=255)                                                                                                                     tGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                <  2:535983/53‑1 (MQ=255)                                                                                                                            |                                                                                                                             CTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAA  >  NC_000913/1006290‑1006537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑