Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,325,247 (T)5→4 coding (502/1896 nt) yciQ → enhancer of membrane protein expression, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,325,2430T.100.0% 167.8 / NA 40coding (498/1896 nt)yciQenhancer of membrane protein expression, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (18/22);  total (18/22)

TTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGAGGACGGAAGAATTGAATCCCGCG  >  NC_000913/1325108‑1325367
                                                                                                                                       |                                                                                                                            
tttCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGtttt                                                                                                                           <  2:279605/139‑1 (MQ=255)
     tGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTtctctc                                                                                                                     <  1:898178/139‑1 (MQ=255)
     tGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTtctctc                                                                                                                     <  2:439852/139‑1 (MQ=255)
        cTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAg                                                                                                                  <  2:246057/139‑1 (MQ=255)
        cTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAg                                                                                                                  <  2:153159/139‑1 (MQ=255)
         tCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGt                                                                                                                 >  1:293978/1‑139 (MQ=255)
                     tCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTg                                                                                                     <  2:487437/139‑1 (MQ=255)
                     tCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTg                                                                                                     <  2:930217/139‑1 (MQ=255)
                       aTTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAg                                                                                                                  >  1:911347/1‑124 (MQ=255)
                       aTTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAg                                                                                                                  <  2:911347/124‑1 (MQ=255)
                           tCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGt                                                                                                                              >  2:559820/1‑109 (MQ=255)
                           tCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGt                                                                                                                              <  1:559820/109‑1 (MQ=255)
                                   aTTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTtctctc                                                                                                                     >  2:674465/1‑109 (MQ=255)
                                   aTTATCAGGTTAAAAATGCTTGCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTtctctc                                                                                                                     <  1:674465/109‑1 (MQ=255)
                                      aTCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGc                                                                                    <  2:422362/139‑1 (MQ=255)
                                          ggTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGaaa                                                                                >  1:813053/1‑139 (MQ=255)
                                           gTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAAt                                                                               >  1:923312/1‑139 (MQ=255)
                                                           tGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGga                                                               <  2:813053/139‑1 (MQ=255)
                                                            gCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCg                                                                             <  2:185210/124‑1 (MQ=255)
                                                            gCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCg                                                                             >  1:185210/1‑124 (MQ=255)
                                                               tGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTtctctc                                                                                                                     <  2:514426/81‑1 (MQ=255)
                                                               tGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTtctctc                                                                                                                     >  1:514426/1‑81 (MQ=255)
                                                                 aGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGagaa                                                            <  2:20704/136‑1 (MQ=255)
                                                                 aGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGagaa                                                            >  1:20704/1‑136 (MQ=255)
                                                                    gggATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGc                                                      <  1:158013/139‑1 (MQ=255)
                                                                       aTTCTGATCTGCTAGTCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac                                                   <  1:327519/139‑1 (MQ=255)
                                                                       aTTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACGCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac                                                   >  1:652946/1‑139 (MQ=255)
                                                                       aTTATGATCTGCTAATCTGGAACGTTACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac                                                   <  2:652946/139‑1 (MQ=255)
                                                                        ttCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGaca                                                  <  2:923312/139‑1 (MQ=255)
                                                                                  cTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTctct                                                                                                            <  2:49354/71‑1 (MQ=255)
                                                                                  cTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTctct                                                                                                            >  1:49354/1‑71 (MQ=255)
                                                                                  cTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTaa                                        >  2:754002/1‑139 (MQ=255)
                                                                                     aTCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATgg                                     >  1:315212/1‑139 (MQ=255)
                                                                                          gAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCa                                >  2:956027/1‑139 (MQ=255)
                                                                                               tGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGagaaga                                                          >  1:820735/1‑108 (MQ=255)
                                                                                               tGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGagaaga                                                          <  2:820735/108‑1 (MQ=255)
                                                                                                 aCCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGa                                                    <  2:925546/112‑1 (MQ=255)
                                                                                                 aCCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGa                                                    >  1:925546/1‑112 (MQ=255)
                                                                                                 aCCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGa                         >  2:914234/1‑139 (MQ=255)
                                                                                                      tAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAgg                                                        >  1:332085/1‑103 (MQ=255)
                                                                                                      tAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAgg                                                        <  2:332085/103‑1 (MQ=255)
                                                                                                               gCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTACTATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGAGGACGGACGAATTg           >  1:462080/1‑139 (MQ=255)
                                                                                                                        aaTTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGAGGACGGAAGAATTGAATCCcgcg  <  2:315212/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                            
TTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGAGGACGGAAGAATTGAATCCCGCG  >  NC_000913/1325108‑1325367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: