Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,742,229 (A)7→6 coding (817/1149 nt) cfa → cyclopropane fatty acyl phospholipid synthase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,742,2230A.100.0% 168.1 / NA 40coding (811/1149 nt)cfacyclopropane fatty acyl phospholipid synthase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (18/22);  total (18/22)

CTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCT  >  NC_000913/1742085‑1742357
                                                                                                                                          |                                                                                                                                      
cTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGa                                                                                                                                        >  2:809925/1‑139 (MQ=255)
  gAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGaaa                                                                                                                                      <  1:787531/139‑1 (MQ=255)
   aaCGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGaaaa                                                                                                                                     >  1:577707/1‑139 (MQ=255)
   aaCGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGaaaa                                                                                                                                     >  2:927633/1‑139 (MQ=255)
                         tttCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATccc                                                                                                              <  2:577707/139‑1 (MQ=255)
                           tCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGAt                                                                                                                             <  2:284588/122‑1 (MQ=255)
                           tCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGAt                                                                                                                             >  1:284588/1‑122 (MQ=255)
                             tgtgGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGa                                                                                                          <  1:922333/139‑1 (MQ=255)
                               tgGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAACCCGATCTGAATGtt                                                                                                                    >  1:468845/1‑127 (MQ=255)
                               tgGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGtt                                                                                                                    <  2:468845/127‑1 (MQ=255)
                                       ttCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTaataaata                                                                                                >  2:484455/1‑139 (MQ=255)
                                         cGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTTGATCGTAATTTGAAATCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAatata                                                                                              <  1:927633/139‑1 (MQ=255)
                                                 tCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGtt                                                                                                                    >  2:955808/1‑109 (MQ=255)
                                                 tCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGtt                                                                                                                    <  1:955808/109‑1 (MQ=255)
                                                     aCCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACg                                                                                  >  2:501813/1‑139 (MQ=255)
                                                                   aTACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCctct                                                                    >  2:997559/1‑139 (MQ=255)
                                                                      ccTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGAt                                                                                                                             <  2:644702/79‑1 (MQ=255)
                                                                      ccTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGAt                                                                                                                             >  1:644702/1‑79 (MQ=255)
                                                                         aTTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGa                                                                                                          >  1:467131/1‑95 (MQ=255)
                                                                         aTTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGa                                                                                                          <  2:467131/95‑1 (MQ=255)
                                                                               cGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTcc                                                                                       <  1:731450/108‑1 (MQ=255)
                                                                               cGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTcc                                                                                       >  2:731450/1‑108 (MQ=255)
                                                                                ggtggtGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTg                                                       >  2:151957/1‑139 (MQ=255)
                                                                                 gtggtgGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGc                                                      >  1:554548/1‑139 (MQ=255)
                                                                                  tggtggATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCt                                                     <  2:448727/139‑1 (MQ=255)
                                                                                       gATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCt                                                     <  2:587509/134‑1 (MQ=255)
                                                                                       gATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGATGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTATGTACGCCAGATTGCt                                                     >  1:587509/1‑134 (MQ=255)
                                                                                            tAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATtaat                                                                                                     >  1:737044/1‑81 (MQ=255)
                                                                                            tAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATtaat                                                                                                     <  2:737044/81‑1 (MQ=255)
                                                                                              aTTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGaa                                         <  1:484455/139‑1 (MQ=255)
                                                                                                          aaGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATcc                                                                                                               <  2:829522/57‑1 (MQ=255)
                                                                                                          aaGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAACAGATCTGAATGTTGATcc                                                                                                               >  1:829522/1‑57 (MQ=255)
                                                                                                             gCATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATg                          <  1:50664/139‑1 (MQ=255)
                                                                                                              cATATTCCTGCTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATgg                         <  1:301999/139‑1 (MQ=255)
                                                                                                                        cTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTtgcctgcc                                                                        <  1:179951/82‑1 (MQ=255)
                                                                                                                        cTCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTtgcctgcc                                                                        >  2:179951/1‑82 (MQ=255)
                                                                                                                         tCCATACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCAt              <  2:554548/139‑1 (MQ=255)
                                                                                                                             tACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGc                                            >  1:542877/1‑105 (MQ=255)
                                                                                                                             tACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGc                                            <  2:542877/105‑1 (MQ=255)
                                                                                                                             tACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCa                                    >  1:177463/1‑113 (MQ=255)
                                                                                                                             tACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCa                                    <  2:177463/113‑1 (MQ=255)
                                                                                                                             tACTATCGGTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACt          >  1:303609/1‑139 (MQ=255)
                                                                                                                                 aTCGGTCCGAAAAAACCGATCTGAATGTTGAGCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCGCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGCAAGACTGGCATAACTTCgg      <  2:672558/139‑1 (MQ=255)
                                                                                                                                     gTTCGAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCt  <  1:997559/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
CTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGCATATTCCTGCTCCATACTATCGGTTCGAAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCT  >  NC_000913/1742085‑1742357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: