Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 183,607 Δ1 bp coding (1145/1158 nt) cdaR → carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913183,6070A.98.2% 222.7 / ‑3.1 54coding (1145/1158 nt)cdaRcarbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (31/22);  minor base C (1/0);  total (32/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAACGTCAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGG  >  NC_000913/183477‑183737
                                                                                                                                  |                                                                                                                                  
gCAACGTCAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCg                                                                                                                           <  2:96325/139‑1 (MQ=255)
  aaCGTCAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGt                                                                                                                         <  2:167078/139‑1 (MQ=255)
  aaCGTCAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGt                                                                                                                         >  1:993703/1‑139 (MQ=255)
                           cGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGCTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggcgg                                                                                                >  2:635862/1‑139 (MQ=255)
                                  cccTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                         <  2:993703/139‑1 (MQ=255)
                                     tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                      >  1:317353/1‑139 (MQ=255)
                                     tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                      >  2:159297/1‑139 (MQ=255)
                                     tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTCAAGGtt                                                                                                       <  2:597631/122‑1 (MQ=255)
                                     tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTCAAGGtt                                                                                                       >  1:597631/1‑122 (MQ=255)
                                        aGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                         <  1:791906/133‑1 (MQ=255)
                                        aGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                         >  2:791906/1‑133 (MQ=255)
                                        aGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTc                                                                                   >  1:857162/1‑139 (MQ=255)
                                            tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                              <  2:734779/124‑1 (MQ=255)
                                            tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                              >  1:734779/1‑124 (MQ=255)
                                            tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                               >  1:806516/1‑139 (MQ=255)
                                            tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                               >  1:969351/1‑139 (MQ=255)
                                            tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                               >  2:668973/1‑139 (MQ=255)
                                              ggCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCa                                                                             <  2:857162/139‑1 (MQ=255)
                                                   attcttataTCGGAACTGACCTGGCTTGATTTGGGCAATTTTGTTGACAGGTCGCTGTTGTATGTGGCCTTACAACTGGTGACGAGCGGTAACTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                        <  1:751734/134‑1 (MQ=255)
                                                    aTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                        <  1:553148/138‑1 (MQ=255)
                                                    aTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                        >  2:553148/1‑138 (MQ=255)
                                                          tatCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTa                                                                 >  2:366297/1‑139 (MQ=255)
                                                               gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                   >  2:229844/1‑84 (MQ=255)
                                                               gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                   <  1:229844/84‑1 (MQ=255)
                                                                  cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGaa                                                         >  1:973856/1‑139 (MQ=255)
                                                                     aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGTGGTAAGTTATGCGTTAAGGTTGAgg                                                                                                   <  1:85088/94‑1 (MQ=255)
                                                                     aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAgg                                                                                                   >  2:85088/1‑94 (MQ=255)
                                                                     aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGgcgc                                                                                             <  1:938491/100‑1 (MQ=255)
                                                                     aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGgcgc                                                                                             >  2:938491/1‑100 (MQ=255)
                                                                     aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                      >  2:701878/1‑123 (MQ=255)
                                                                     aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                      <  1:701878/123‑1 (MQ=255)
                                                                     aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                      >  2:438322/1‑139 (MQ=255)
                                                                     aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCAGCGCTGGCTCATTCCCCTCAGCCCg                                                                        <  2:361174/121‑1 (MQ=255)
                                                                     aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCAGCGCTGGCTCATTCCCCTCAGCCCg                                                                        >  1:361174/1‑121 (MQ=255)
                                                                       cGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGccc                                                    >  2:885877/1‑139 (MQ=255)
                                                                          gCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGc                                                                           >  2:512161/1‑113 (MQ=255)
                                                                          gCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGc                                                                           <  1:512161/113‑1 (MQ=255)
                                                                                  tGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                         <  2:969351/139‑1 (MQ=255)
                                                                                    ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                       >  1:757211/1‑139 (MQ=255)
                                                                                    ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                       >  2:526665/1‑139 (MQ=255)
                                                                                       aaTTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                    <  1:885877/139‑1 (MQ=255)
                                                                                          tttGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCgg                                 <  1:526665/139‑1 (MQ=255)
                                                                                              atgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGcc                                                     >  2:993969/1‑115 (MQ=255)
                                                                                              atgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGcc                                                     <  1:993969/115‑1 (MQ=255)
                                                                                                   aGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGATGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTa                        >  2:103191/1‑139 (MQ=255)
                                                                                                    ggTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAc                       >  1:333989/1‑139 (MQ=255)
                                                                                                            ctgTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAt                                                                                     <  2:182763/69‑1 (MQ=255)
                                                                                                            ctgTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAt                                                                                     >  1:182763/1‑69 (MQ=255)
                                                                                                                    ggCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAg       >  1:459727/1‑139 (MQ=255)
                                                                                                                      cGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCggg                                <  2:467678/112‑1 (MQ=255)
                                                                                                                      cGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCggg                                >  1:467678/1‑112 (MQ=255)
                                                                                                                         tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGaa                                                 >  2:746095/1‑92 (MQ=255)
                                                                                                                         tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGaa                                                 <  1:746095/92‑1 (MQ=255)
                                                                                                                         tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg  >  1:635420/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                  
GCAACGTCAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGG  >  NC_000913/183477‑183737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: