Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,134,811 Δ1 bp coding (681/693 nt) yghT → putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,134,8110T.96.9% 126.5 / ‑2.8 32coding (681/693 nt)yghTputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (13/18);  minor base C (1/0);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAA  >  NC_000913/3134686‑3134936
                                                                                                                             |                                                                                                                             
aaGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATc                                                                                                                 <  1:266305/139‑1 (MQ=255)
                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                <  1:258715/139‑1 (MQ=255)
                             tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGc                                                                                                   <  1:775144/124‑1 (MQ=255)
                             tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGc                                                                                                   >  2:775144/1‑124 (MQ=255)
                                tggtgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                 <  1:457265/139‑1 (MQ=255)
                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                >  2:754974/1‑111 (MQ=255)
                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                <  1:754974/111‑1 (MQ=255)
                                                  tatCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGTAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                               >  1:457942/1‑139 (MQ=255)
                                                  tatCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                               >  2:898638/1‑139 (MQ=255)
                                                       aTGCCAATCAGCCACTGGAATAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATc                                                                                                                 >  1:595014/1‑84 (MQ=255)
                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATc                                                                                                                 <  2:595014/84‑1 (MQ=255)
                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                          >  2:419515/1‑139 (MQ=255)
                                                            aaTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGTAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                     <  2:457942/139‑1 (MQ=255)
                                                                     cTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                     >  1:307057/1‑98 (MQ=255)
                                                                     cTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                     <  2:307057/98‑1 (MQ=255)
                                                                         aaCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTa                                        <  1:12445/139‑1 (MQ=255)
                                                                            aGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                     <  1:749215/91‑1 (MQ=255)
                                                                            aGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCCGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                     >  2:749215/1‑91 (MQ=255)
                                                                                gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                            <  2:764635/112‑1 (MQ=255)
                                                                                gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                            >  1:764635/1‑112 (MQ=255)
                                                                                   ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                     >  2:17340/1‑84 (MQ=255)
                                                                                   ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                     <  1:17340/84‑1 (MQ=255)
                                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCTCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                         >  1:600094/1‑139 (MQ=255)
                                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGcc                                                                                                  >  2:161053/1‑66 (MQ=255)
                                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGcc                                                                                                  <  1:161053/66‑1 (MQ=255)
                                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                     >  2:80687/1‑111 (MQ=255)
                                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                     <  1:80687/111‑1 (MQ=255)
                                                                                                         aCGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta        <  1:898638/139‑1 (MQ=255)
                                                                                                          cGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa       <  1:419515/139‑1 (MQ=255)
                                                                                                               tttATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTTAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaagtaa  <  2:600094/139‑1 (MQ=255)
                                                                                                               tttATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                  <  1:949161/91‑1 (MQ=255)
                                                                                                               tttATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                  >  2:949161/1‑91 (MQ=255)
                                                                                                                             |                                                                                                                             
AAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAA  >  NC_000913/3134686‑3134936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: