Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 401,548 (G)5→4 coding (163/261 nt) iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation, anti‑adapter protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913401,5440G.100.0% 140.6 / NA 34coding (159/261 nt)iraPanti‑RssB factor, RpoS stabilzer during Pi starvation, anti‑adapter protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (17/17);  total (17/17)

TTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NC_000913/401410‑401678
                                                                                                                                      |                                                                                                                                      
ttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggc                                                                                                                                    >  2:833458/1‑138 (MQ=255)
 tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   <  1:105323/139‑3 (MQ=255)
       aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTAc                                                                                                                            <  2:823248/139‑1 (MQ=255)
       aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTAc                                                                                                                            >  2:542290/1‑139 (MQ=255)
             cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTa                                                                                                                      >  1:256428/1‑139 (MQ=255)
             cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTa                                                                                                                      >  2:327266/1‑139 (MQ=255)
                          agagTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCa                                                                                                         <  2:577931/139‑1 (MQ=255)
                                          tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcgct                                                                                                                                 >  2:778297/1‑96 (MQ=255)
                                          tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcgct                                                                                                                                 <  1:778297/100‑5 (MQ=255)
                                            tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTTCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       >  2:494978/1‑139 (MQ=255)
                                                  ggTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgc                                                                                 >  1:311890/1‑139 (MQ=255)
                                                         gCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTgcg                                                                          >  2:414549/1‑139 (MQ=255)
                                                           tttGGAGATTATCGTCACTTCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGcgcg                                                                        <  1:494978/139‑1 (MQ=255)
                                                           tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGc                                                                                                       <  1:132500/108‑1 (MQ=255)
                                                           tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGc                                                                                                       >  2:132500/1‑108 (MQ=255)
                                                            ttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGa                                                                       <  2:712506/139‑1 (MQ=255)
                                                            ttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGa                                                                       <  1:542290/139‑1 (MQ=255)
                                                             tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  1:66932/1‑139 (MQ=255)
                                                             tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:809385/1‑139 (MQ=255)
                                                             tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  1:237367/1‑139 (MQ=255)
                                                             tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:416035/1‑139 (MQ=255)
                                                             tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:416034/1‑139 (MQ=255)
                                                                            cTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGAt                                                                                                             >  2:939474/1‑85 (MQ=255)
                                                                            cTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGAt                                                                                                             <  1:939474/85‑1 (MQ=255)
                                                                            cTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTa                                                       <  1:416035/139‑1 (MQ=255)
                                                                            cTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTa                                                       <  1:416034/139‑1 (MQ=255)
                                                                                    cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCa                                               <  1:833458/139‑1 (MQ=255)
                                                                                       cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                  >  1:160559/1‑133 (MQ=255)
                                                                                       cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                  <  2:160559/133‑1 (MQ=255)
                                                                                                gcgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTa                                   <  2:66932/139‑1 (MQ=255)
                                                                                                 cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   >  2:314810/1‑41 (MQ=255)
                                                                                                 cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   <  1:314810/43‑3 (MQ=255)
                                                                                                 cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                               >  1:504403/1‑94 (MQ=255)
                                                                                                 cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                               <  2:504403/94‑1 (MQ=255)
                                                                                                      aaTGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgac                                                                                              <  2:494168/74‑1 (MQ=255)
                                                                                                      aaTGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgac                                                                                              >  1:494168/1‑74 (MQ=255)
                                                                                                                  cGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCaa                 <  1:809385/139‑1 (MQ=255)
                                                                                                                     ttgattgaTCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:884477/139‑1 (MQ=255)
                                                                                                                     ttgattgaTCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:311890/139‑1 (MQ=255)
                                                                                                                                 gTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGt  >  1:133862/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NC_000913/401410‑401678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: