Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 7,925 +A coding (35/1431 nt) yaaJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009137,9221.A97.1% 212.5 / ‑0.7 68coding (38/1431 nt)yaaJputative transporter
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base A (31/35);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTC  >  NC_000913/7791‑8054
                                                                                                                                     |                                                                                                                                     
cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCaaaagga                                                                                                                               <  2:305201/139‑5 (MQ=255)
  ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              <  2:805360/139‑1 (MQ=255)
  ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              >  1:351324/1‑139 (MQ=255)
   cTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                             >  1:472319/1‑139 (MQ=255)
   cTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                             >  2:156303/1‑139 (MQ=255)
           ggATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATg                                                                                                                     >  2:722343/1‑139 (MQ=255)
            gATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGa                                                                                                                    >  2:705582/1‑139 (MQ=255)
             aTGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGaa                                                                                                                   <  1:156303/139‑1 (MQ=255)
                aaTGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGAGTAACTGCACAAATCCGGTGCGAAAAGTGAACCAACGACCTGCGCCGATGATCAGGTAAATCATTACCGTTCCGCAAAAGGACGCTGTTAaggaagga                                                                                                                >  1:373963/1‑139 (MQ=255)
                            aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCGACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                    <  1:722343/139‑1 (MQ=255)
                            aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                    <  2:30214/139‑1 (MQ=255)
                                 ttttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCt                                                                                                                            >  2:1008292/1‑110 (MQ=255)
                                 ttttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCt                                                                                                                            <  1:1008292/110‑1 (MQ=255)
                                  tttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                              >  2:487052/1‑139 (MQ=255)
                                   ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat                                                                                             >  2:791249/1‑139 (MQ=255)
                                   ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCCGgcatgcat                                                                                             >  2:650430/1‑139 (MQ=255)
                                     gCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCatat                                                                                           <  2:205342/139‑1 (MQ=255)
                                       cAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATcc                                                                                         <  1:791249/139‑1 (MQ=255)
                                                   tGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCg                                                                             >  1:417437/1‑139 (MQ=255)
                                                      aGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTc                                                                          >  1:426805/1‑139 (MQ=255)
                                                        cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTcgc                                                                        >  2:373347/1‑139 (MQ=255)
                                                          aaaCTGCATAAATCCGTTGCGCAAAGTGAACGAACAACCAGCGGCGAATAGCAGGTAAATCAATACCGATCCCCAAGAGGACGGAGCTAATGACGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa                                                                      <  1:775593/139‑1 (MQ=255)
                                                          aaaCTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa                                                                      <  1:554090/139‑1 (MQ=255)
                                                            aCTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATcc                                                                                         <  1:279085/118‑1 (MQ=255)
                                                            aCTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATcc                                                                                         >  2:279085/1‑118 (MQ=255)
                                                                cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACtt                                                                >  1:177023/1‑139 (MQ=255)
                                                                  caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                              <  2:426805/139‑1 (MQ=255)
                                                                  caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                              <  2:472319/139‑1 (MQ=255)
                                                                     aTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                             <  2:149154/73‑1 (MQ=255)
                                                                     aTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                             >  1:149154/1‑73 (MQ=255)
                                                                       ccGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCtgtg                                                         <  2:351324/139‑1 (MQ=255)
                                                                         ggTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATTCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTa                                                       <  1:373347/139‑1 (MQ=255)
                                                                         ggTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTa                                                       <  2:40529/139‑1 (MQ=255)
                                                                             cGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATc                                                                                          >  1:747794/1‑100 (MQ=255)
                                                                             cGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATc                                                                                          <  2:747794/100‑1 (MQ=255)
                                                                             cGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACg                                                   <  1:433204/139‑1 (MQ=255)
                                                                             cGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACg                                                   >  2:646297/1‑139 (MQ=255)
                                                                                    tGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCaa                                            <  2:177023/139‑1 (MQ=255)
                                                                                     gAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                              >  2:965079/1‑120 (MQ=255)
                                                                                     gAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                              <  1:965079/120‑1 (MQ=255)
                                                                                     gAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAGACGTTACCAAt                                           <  1:482690/139‑1 (MQ=255)
                                                                                     gAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAAt                                           <  1:457908/139‑1 (MQ=255)
                                                                                       aCCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                              <  1:387832/118‑1 (MQ=255)
                                                                                       aCCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                              >  2:387832/1‑118 (MQ=255)
                                                                                        ccAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGt                                        >  1:932227/1‑139 (MQ=255)
                                                                                          aacaacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGttt                                      <  1:646297/139‑1 (MQ=255)
                                                                                           acaacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTa                                     >  1:159027/1‑139 (MQ=255)
                                                                                            caacCTGCGCCGAAGAGCAAGTAAATCATTACCGATCCCCAAAAGGAAGCTGTTAATGAAGGAGAAAAAATGTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTGCATGTGTAAACGTTACCAAATGTTTaa                                    >  1:357698/1‑139 (MQ=255)
                                                                                              acCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaaga                                  <  1:400922/139‑1 (MQ=255)
                                                                                              acCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaaga                                  <  2:932227/139‑1 (MQ=255)
                                                                                                   cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              <  2:6112/42‑1 (MQ=255)
                                                                                                   cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                              >  1:6112/1‑42 (MQ=255)
                                                                                                   cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAATGAGAAAAAATCTgg                                                                                                    <  1:221352/68‑1 (MQ=255)
                                                                                                   cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                    <  1:540635/68‑1 (MQ=255)
                                                                                                   cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                    >  2:221352/1‑68 (MQ=255)
                                                                                                   cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                    >  2:540635/1‑68 (MQ=255)
                                                                                                   cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCtgtg                                                         <  2:369098/111‑1 (MQ=255)
                                                                                                   cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCtgtg                                                         >  1:369098/1‑111 (MQ=255)
                                                                                                     ccGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGtatat                           <  1:487052/139‑1 (MQ=255)
                                                                                                          gagCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGtata                            <  2:20638/133‑1 (MQ=255)
                                                                                                          gagCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGtata                            >  1:20638/1‑133 (MQ=255)
                                                                                                                   aaTCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTAc             <  2:417437/139‑1 (MQ=255)
                                                                                                                          aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                              <  2:630900/83‑1 (MQ=255)
                                                                                                                          aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                              >  1:630900/1‑83 (MQ=255)
                                                                                                                             atccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCg                                                                             <  2:734247/61‑1 (MQ=255)
                                                                                                                             atccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTc  >  2:485791/4‑139 (MQ=255)
                                                                                                                              aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCg                                                                             >  1:734247/1‑64 (MQ=255)
                                                                                                                              aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaag                                   >  1:222903/1‑106 (MQ=255)
                                                                                                                              aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaag                                   <  2:222903/106‑1 (MQ=255)
                                                                                                                               tCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAAt                                           >  2:181813/1‑97 (MQ=255)
                                                                                                                               tCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAAt                                           <  1:181813/97‑1 (MQ=255)
                                                                                                                               ccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACtt                                                                <  1:860145/74‑1 (MQ=255)
                                                                                                                               ccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACtt                                                                >  2:860145/2‑75 (MQ=255)
                                                                                                                               ccccaAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTAc             <  1:876431/125‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTC  >  NC_000913/7791‑8054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: