Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,741,640 (T)6→7 coding (228/1149 nt) cfa → cyclopropane fatty acyl phospholipid synthase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,741,6341.T100.0% 118.5 / NA 38M74I (ATG→ATTcfacyclopropane fatty acyl phospholipid synthase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (18/20);  total (18/20)

TATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGC  >  NC_000913/1741496‑1741767
                                                                                                                                           |                                                                                                                                     
tataGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATg                                                                                                                                        >  2:216161/1‑139 (MQ=255)
          aaCGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGtttttttagc                                                                                                                             >  2:631100/1‑135 (MQ=255)
                  tGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCtt                                                                                                                      <  1:631100/139‑1 (MQ=255)
                     cccGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTAcg                                                                                                                   <  1:216161/139‑1 (MQ=255)
                       cggcggATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTAcgcg                                                                                                                 <  1:406281/139‑1 (MQ=255)
                              tatTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGtct                                                                                                          >  2:141812/1‑139 (MQ=255)
                                   gtgtGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTATGCGCAGGTCTCgaga                                                                                                     <  2:92668/139‑1 (MQ=255)
                                   gtgtGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCgaga                                                                                                     <  1:916955/139‑1 (MQ=255)
                                                  aTTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCcatc                                                                                      <  2:305438/139‑1 (MQ=255)
                                                                        agaagGCTCTTTGGGGGTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTAt                                                                >  2:573397/1‑139 (MQ=255)
                                                                              ctctTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACgcgc                                                                                                                <  1:255602/85‑1 (MQ=255)
                                                                              ctctTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACgcgc                                                                                                                >  2:255602/1‑85 (MQ=255)
                                                                              ctctTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCgg                                                          >  1:534029/1‑139 (MQ=255)
                                                                                ctTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCa                                                                                                 <  2:969561/98‑1 (MQ=255)
                                                                                ctTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCa                                                                                                 >  1:969561/1‑98 (MQ=255)
                                                                                          aGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTAcgcg                                                                                                                 <  1:704321/72‑1 (MQ=255)
                                                                                          aGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTAcgcg                                                                                                                 >  2:704321/1‑72 (MQ=255)
                                                                                           ggCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGtct                                                                                                          <  2:5808/78‑1 (MQ=255)
                                                                                           ggCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGtct                                                                                                          >  1:5808/1‑78 (MQ=255)
                                                                                            gCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCa                                            <  1:130636/139‑1 (MQ=255)
                                                                                                      tatGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCagag                                  >  1:963387/1‑139 (MQ=255)
                                                                                                             ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCca                                                                                        <  2:462104/78‑1 (MQ=255)
                                                                                                             ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCca                                                                                        >  1:462104/1‑78 (MQ=255)
                                                                                                             ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTaaaaaa                           >  1:247592/1‑139 (MQ=255)
                                                                                                             ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTaaaaaa                           >  1:1006787/1‑139 (MQ=255)
                                                                                                             ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTaaaaaa                           >  2:50664/1‑139 (MQ=255)
                                                                                                             ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTaaaaaa                           <  2:467478/139‑1 (MQ=255)
                                                                                                             ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTaaaaaa                           >  1:467478/1‑139 (MQ=255)
                                                                                                                  gtggGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGc                      >  2:220301/1‑139 (MQ=255)
                                                                                                                        aTGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGAt                >  1:662663/1‑139 (MQ=255)
                                                                                                                        aTGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGAt                <  2:963387/139‑1 (MQ=255)
                                                                                                                         tgtgACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCag                                    >  2:158215/1‑118 (MQ=255)
                                                                                                                         tgtgACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCag                                    <  1:158215/118‑1 (MQ=255)
                                                                                                                           tgACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATGATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGt             <  1:929438/139‑1 (MQ=255)
                                                                                                                             aCCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCg           >  1:687099/1‑139 (MQ=255)
                                                                                                                                  cTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCaaa      <  2:247592/139‑1 (MQ=255)
                                                                                                                                  cTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGATCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCaaa      <  2:534029/139‑1 (MQ=255)
                                                                                                                                     gATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTg                                       >  1:912198/1‑103 (MQ=255)
                                                                                                                                     gATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTg                                       <  2:912198/103‑1 (MQ=255)
                                                                                                                                      atatGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGc  <  2:761935/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
TATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGC  >  NC_000913/1741496‑1741767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: