| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,950,559 | (C)5→6 | intergenic (‑37/‑273) | aspS ← / → yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,950,554 | 1 | . | C | 98.0% | 172.9 / ‑3.3 | 49 | intergenic (‑32/‑278) | aspS/yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (25/23); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
AAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑C‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAC > NC_000913/1950419‑1950683 | aaGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑c‑cc < 2:10000/139‑1 (MQ=255) cAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAg > 2:952063/1‑139 (MQ=255) acgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGc < 2:736103/139‑1 (MQ=255) tGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAAGTTAGCTGCCTGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 1:622770/139‑1 (MQ=255) aaCCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg < 1:952063/139‑1 (MQ=255) cacacaGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTAt > 1:410315/1‑139 (MQ=255) gTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt > 1:1745/1‑122 (MQ=255) gTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 2:1745/122‑1 (MQ=255) tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 2:28107/1‑139 (MQ=255) tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 1:198829/1‑139 (MQ=255) cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACtg > 2:958505/1‑139 (MQ=255) tgcCCCACGTGGGACAAACGGAGCTGTCTACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCa < 1:688998/94‑1 (MQ=255) tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCa > 2:688998/1‑94 (MQ=255) tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 2:430065/113‑1 (MQ=255) tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt > 1:430065/1‑113 (MQ=255) tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGaaa > 1:904568/1‑139 (MQ=255) cACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCctgctg > 1:732186/1‑85 (MQ=255) cACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCctgctg < 2:732186/85‑1 (MQ=255) aCGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:28107/139‑1 (MQ=255) aCGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 2:904568/139‑1 (MQ=255) aCGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:874985/139‑1 (MQ=255) cGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt > 1:703654/1‑106 (MQ=255) cGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 2:703654/106‑1 (MQ=255) gTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggcgg < 2:198829/139‑1 (MQ=255) gggACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCt > 2:327322/1‑139 (MQ=255) aCGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATAc > 2:957971/1‑139 (MQ=255) cGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTTCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAGTGAAAAAAGGAGGCTATTATACt > 1:361914/1‑139 (MQ=255) tCCACAATCTTCTGTACGCATGAGATCTCCCTTAACTTAGCTGCCGGCGGATG‑CTCCCCTGATGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACGGAATGAAAAAAGGCGGCTTTTATACTGGAAATTc > 1:905051/1‑139 (MQ=255) tCCACAATATTCTGTACGCATGAGATATCTCTTAACTTAGCTGCCAGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg > 1:583110/1‑90 (MQ=255) tCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg < 2:583110/90‑1 (MQ=255) acaATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGt > 2:513535/1‑95 (MQ=255) acaATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGt < 1:513535/95‑1 (MQ=255) tatTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCa < 2:410315/139‑1 (MQ=255) tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTa > 1:274864/1‑85 (MQ=255) tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTa < 2:274864/85‑1 (MQ=255) tGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa < 1:327322/139‑1 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTc < 2:713374/61‑1 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTc > 1:713374/1‑61 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 1:836862/68‑1 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt > 2:836862/1‑68 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCt > 2:564710/1‑139 (MQ=255) gaTATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGcc < 1:564710/139‑1 (MQ=255) ccTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCt > 1:446016/1‑139 (MQ=255) tAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGa > 1:128436/1‑139 (MQ=255) aaCTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCa < 2:22811/113‑1 (MQ=255) aaCTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCa > 1:22811/1‑113 (MQ=255) aGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCt > 1:278137/1‑139 (MQ=255) cTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCg > 2:243100/1‑139 (MQ=255) ccGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 2:278137/139‑1 (MQ=255) cggcggATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAc < 2:446016/139‑1 (MQ=255) | AAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑C‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAC > NC_000913/1950419‑1950683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |