Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,018,530 (C)6→7 coding (145/1128 nt) fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,018,5241.C100.0% 94.5 / NA 27A47P (GCC→CCC) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (14/13);  total (14/13)

CTTGATTACCGCCGACGTTGACACCACCACATTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCTGTGGATGAAACACCGCCTGTCAT  >  NC_000913/2018403‑2018648
                                                                                                                          |                                                                                                                            
cTTGATTACCGCCGACGTTGACACCACCACATTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCtggt                                                                                                              <  1:496089/139‑1 (MQ=255)
    aTTACCGCCGACGTTGACACCACCACATTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGcccccccag                                                                                                                     <  2:116698/127‑4 (MQ=255)
    aTTACCGCCGACGTTGACACCACCACATTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGcccccccag                                                                                                                     >  1:116698/1‑124 (MQ=255)
                      accaccacATTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGAt                                                                                                    <  2:615471/127‑1 (MQ=255)
                      accaccacATTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGAt                                                                                                    >  1:615471/1‑127 (MQ=255)
                          ccacATTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCaa                                                                                                         >  2:986977/1‑118 (MQ=255)
                          ccacATTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCaa                                                                                                         <  1:986977/118‑1 (MQ=255)
                             caTTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGc                                                                                                                             <  1:3309/94‑1 (MQ=255)
                             caTTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGc                                                                                                                             >  2:3309/1‑94 (MQ=255)
                                  ccTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCa                                                                                                          <  2:529471/109‑1 (MQ=255)
                                  ccTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCa                                                                                                          >  1:529471/1‑109 (MQ=255)
                                     ggcggcAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTg                                                                         >  2:522833/1‑139 (MQ=255)
                                            aaGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCg                                                                  >  2:409079/1‑139 (MQ=255)
                                                           cacaAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGAcg                                                   <  1:409079/139‑1 (MQ=255)
                                                                                     gAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCt                         <  2:458329/139‑1 (MQ=255)
                                                                                         cATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCTGTgg                     >  2:189311/1‑139 (MQ=255)
                                                                                          aTTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCTGTGGa                    <  1:189311/139‑1 (MQ=255)
                                                                                            tAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCTGTGGATg                  >  1:979439/1‑139 (MQ=255)
                                                                                               cAGGCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCTGTGGATGaaa               <  2:979439/139‑1 (MQ=255)
                                                                                                 ggCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGcaaca                                            >  1:730769/1‑108 (MQ=255)
                                                                                                 ggCGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGcaaca                                            <  2:730769/108‑1 (MQ=255)
                                                                                                   cGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAg                                          <  1:733115/108‑1 (MQ=255)
                                                                                                   cGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAg                                          >  2:733115/1‑108 (MQ=255)
                                                                                                   cGAGACAACTACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCTGTGGATGAAACAcc           >  2:232530/1‑139 (MQ=255)
                                                                                                            tACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCaa                                                                                                         <  1:962369/36‑1 (MQ=255)
                                                                                                            tACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCaa                                                                                                         >  2:962369/1‑36 (MQ=37)
                                                                                                            tACCGACAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCTGTGGATGAAACACCGCCTGtcat  >  2:831953/1‑139 (MQ=255)
                                                                                                                 aCAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCTGTGGATGAAACACCGCCt       >  2:282480/1‑129 (MQ=255)
                                                                                                                 aCAAAGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCTGTGGATGAAACACCGCCt       <  1:282480/129‑1 (MQ=255)
                                                                                                                     aGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAAt                                     >  2:213613/1‑95 (MQ=255)
                                                                                                                     aGCGGCCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAAt                                     <  1:213613/95‑1 (MQ=255)
                                                                                                                          |                                                                                                                            
CTTGATTACCGCCGACGTTGACACCACCACATTGCCTGGCGGCAAAGCCAGCGATGCTGCACAAGATTTTCTCGCGTTGTTGAGCGAAGCATTAGCAGGCGAGACAACTACCGACAAAGCGGCCCCCCAGTTGCTGGTGGCAACAGATAAGCCCACGACAAAAGGCGAGCCGCTGATCAGCGATATTGTTTCCGACGCGCAACAAGCTAATTTACTGATCCCTGTGGATGAAACACCGCCTGTCAT  >  NC_000913/2018403‑2018648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: