Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,478 (C)6→7 intergenic (‑132/+13) pncC ← / ← mltB nicotinamide‑nucleotide amidohydrolase, NMN amidohydrolase/membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,4721.C100.0% 128.2 / NA 37intergenic (‑126/+19)pncC/mltBnicotinamide‑nucleotide amidohydrolase, NMN amidohydrolase/membrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (14/23);  total (14/23)

GTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACC  >  NC_000913/2824338‑2824600
                                                                                                                                       |                                                                                                                                
gtcagtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGcccc                                                                                                                              <  2:677565/139‑1 (MQ=255)
 tcagtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGccccc                                                                                                                             >  2:259102/1‑139 (MQ=255)
    gtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGccccccct                                                                                                                          >  2:679098/1‑137 (MQ=255)
      catcaCAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGccccccctct                                                                                                                        <  1:758477/139‑5 (MQ=255)
            aaCCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTaaa                                                                                                                   <  2:635397/139‑1 (MQ=255)
            aaCCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTaaa                                                                                                                   <  2:211225/139‑1 (MQ=255)
                    tcagtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACt                                                                                                           >  1:168565/1‑139 (MQ=255)
                                              aTAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTc                                                                                <  2:486789/139‑1 (MQ=255)
                                              aTAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTc                                                                                <  2:168565/139‑1 (MQ=255)
                                                  cACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGccccccct                                                                                                                          <  1:396171/93‑3 (MQ=255)
                                                  cACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGccccccct                                                                                                                          >  2:396171/1‑91 (MQ=255)
                                                     tttCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                      <  2:934275/126‑1 (MQ=255)
                                                     tttCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                      >  1:934275/1‑126 (MQ=255)
                                                       tcctCAGGAAGCTGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAGAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                       >  1:585248/4‑139 (MQ=255)
                                                       tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                       >  2:965452/1‑139 (MQ=255)
                                                                   tGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTa                                                                                                                     >  1:127606/1‑82 (MQ=255)
                                                                   tGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTa                                                                                                                     <  2:127606/82‑1 (MQ=255)
                                                                   tGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTg                                                           <  1:965452/139‑1 (MQ=255)
                                                                    ggTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTa                                                                                                                     <  1:910379/81‑1 (MQ=255)
                                                                    ggTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTa                                                                                                                     >  2:910379/1‑81 (MQ=255)
                                                                         tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCt                                                                              >  1:135609/1‑114 (MQ=255)
                                                                         tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCt                                                                              <  2:135609/114‑1 (MQ=255)
                                                                         tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGcccccc                                                                                                                            <  1:494756/68‑1 (MQ=255)
                                                                         tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGcccccc                                                                                                                            >  2:494756/1‑68 (MQ=255)
                                                                            cGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                  >  1:254690/1‑139 (MQ=255)
                                                                               tGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGc                                               <  2:585248/139‑1 (MQ=255)
                                                                                gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                              <  1:679098/139‑1 (MQ=255)
                                                                                 cAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtg                                             <  1:259102/139‑1 (MQ=255)
                                                                                      ttACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGtt                                        <  2:86486/139‑1 (MQ=255)
                                                                                                gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                              <  1:701035/123‑1 (MQ=255)
                                                                                                gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                              >  2:701035/1‑123 (MQ=255)
                                                                                                  ttCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGAt                            <  2:765829/139‑1 (MQ=255)
                                                                                                          aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTa                                                                             <  2:948840/82‑1 (MQ=255)
                                                                                                          aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTa                                                                             >  1:948840/1‑82 (MQ=255)
                                                                                                           aaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCa                                                              >  2:459067/1‑96 (MQ=255)
                                                                                                           aaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCa                                                              <  1:459067/96‑1 (MQ=255)
                                                                                                            aGCTGATTAGCCAGAGGGAAGCTCACGccccccct                                                                                                                          >  2:399000/1‑33 (MQ=255)
                                                                                                            aGCTGATTAGCCAGAGGGAAGCTCACGccccccct                                                                                                                          <  1:399000/35‑3 (MQ=255)
                                                                                                               tGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCgcgccagcgcc                                                                                          >  2:50711/1‑64 (MQ=255)
                                                                                                               tGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCgcgccagcgcc                                                                                          <  1:50711/64‑1 (MQ=255)
                                                                                                                 aTTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCgg             <  2:552668/139‑1 (MQ=255)
                                                                                                                            ggAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGGCCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAcc  <  1:263318/139‑1 (MQ=255)
                                                                                                                            ggAAGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAcc  <  2:254690/139‑1 (MQ=255)
                                                                                                                               aGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                              >  1:475375/1‑92 (MQ=255)
                                                                                                                               aGCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                              <  2:475375/92‑1 (MQ=255)
                                                                                                                                gCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTa    >  2:404530/1‑133 (MQ=255)
                                                                                                                                gCTCACGCCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTa    <  1:404530/133‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                
GTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACC  >  NC_000913/2824338‑2824600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: