| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,400,615 | (T)7→8 | intergenic (+19/‑57) | hfq → / → hflX | global sRNA chaperone, HF‑I, host factor for RNA phage Q beta replication/GTPase, stimulated by 50S subunit binding, Mn(2+) homeostasis regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,400,608 | 1 | . | T | 95.9% | 142.1 / ‑4.5 | 50 | intergenic (+12/‑64) | hfq/hflX | global sRNA chaperone, HF‑I, host factor for RNA phage Q beta replication/GTPase, stimulated by 50S subunit binding, Mn(2+) homeostasis regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base T (22/25); minor base C (1/1); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTG‑TTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAA > NC_000913/4400471‑4400737 | gttgtCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTG‑t > 2:220307/1‑139 (MQ=255) tgtCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTG‑ttt > 1:272911/1‑139 (MQ=255) ccGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTG‑ttttttt < 1:220307/139‑1 (MQ=255) caGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGTAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTg < 2:537538/139‑1 (MQ=255) cGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGa > 2:913053/1‑117 (MQ=255) cGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGa < 1:913053/117‑1 (MQ=255) cGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCg < 1:379679/139‑1 (MQ=255) ccGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCt < 1:128726/139‑1 (MQ=255) ccGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCt < 2:272911/139‑1 (MQ=255) ggTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATc < 1:1007436/123‑1 (MQ=255) ggTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATc > 2:1007436/1‑123 (MQ=255) tGGCGGTACCAGCAGTTACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGCTCCTGCGTTCCCCGCTGATc < 2:684938/139‑1 (MQ=255) aCTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCt < 2:739028/118‑1 (MQ=255) aCTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCt > 1:739028/1‑118 (MQ=255) aCTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATAc > 2:463488/1‑139 (MQ=255) catcatGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACgg > 1:229920/1‑86 (MQ=255) catcatGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACgg < 2:229920/86‑1 (MQ=255) tGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGa < 2:485551/139‑1 (MQ=255) ggTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGAc < 1:614523/139‑1 (MQ=255) gTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCt < 1:833260/106‑1 (MQ=255) gTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCt > 2:833260/1‑106 (MQ=255) gTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGAcc > 2:912209/1‑139 (MQ=255) agcagcGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGt > 2:685352/1‑139 (MQ=255) agcagcGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATATATTTAGAGGGTTATACGCTTGTTTGACCGt > 1:80151/1‑139 (MQ=255) agcagcGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTG‑TTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGtt > 2:777846/1‑139 (MQ=255) gcagcGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTaga > 1:523897/1‑115 (MQ=255) gcagcGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTaga < 2:523897/115‑1 (MQ=255) cgcgcAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCt > 2:651266/1‑87 (MQ=255) cgcgcAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCt < 1:651266/87‑1 (MQ=255) cgcgcAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTa < 2:683108/136‑1 (MQ=255) cgcgcAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTa > 1:683108/1‑136 (MQ=255) gcgcAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTatgat > 1:856767/1‑139 (MQ=255) cgcAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGa < 2:964926/73‑1 (MQ=255) cgcAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGa > 1:964926/1‑73 (MQ=255) cgcAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCg > 1:518749/1‑95 (MQ=255) cgcAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCg < 2:518749/95‑1 (MQ=255) cgcAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTatgatg > 1:153011/1‑139 (MQ=255) gAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTgg > 1:820284/1‑139 (MQ=255) aCTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAg < 2:153011/139‑1 (MQ=255) ttCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCa < 1:685352/139‑1 (MQ=255) cgcgCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGc < 1:912209/139‑1 (MQ=255) cgcAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCgg > 2:271930/1‑139 (MQ=255) cAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTa < 2:820284/139‑1 (MQ=255) cAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTa > 1:945113/1‑139 (MQ=255) cAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATAc < 2:999838/98‑1 (MQ=255) cAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATAc > 1:999838/1‑98 (MQ=255) gACAGCGAAGAAGCCGCATACGGTTTCCGGCTGCTCTTTTTACACGGGTAGCCAGCGATCCTGCGTTGCCCGCTGCTCTATCCAGAGGGCTATACGCTAGTTTGACCGTTATGATGCTGGTCACCAGGCGCCACTggca > 1:246881/1‑137 (MQ=255) cGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCt > 2:35362/1‑69 (MQ=255) cGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCt < 1:35362/69‑1 (MQ=255) cGAAGAAACCGAATAAGGTTTCGGGCTGCTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACAt < 2:878272/139‑1 (MQ=255) gAATAAGGTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTATTTTACg < 1:463488/139‑1 (MQ=255) gTTTCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACa > 1:289516/1‑139 (MQ=255) ttCGGGCTGTTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACaaa < 2:289516/139‑1 (MQ=255) | GTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTG‑TTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAA > NC_000913/4400471‑4400737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |