Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	376,245	(G)5→6	coding (787/1212 nt)	mhpT →	3‑hydroxyphenylpropionic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	376,240	1	.	G	100.0%	120.3 / NA	34	M261R (ATG→AGG)	mhpT	3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (17/17);  total (17/17)

CTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAAT‑GGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGT  >  NC_000913/376113‑376359                                                                                                                                 |                                                                                                                        ctgctgTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCa                                                                                                               >  1:679603/1‑139 (MQ=255)         gTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGAc                                                                                                       >  1:73740/1‑139 (MQ=255)           tgtgTTATTTCTTCACTCAGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGt                                                                                                     <  2:679603/139‑1 (MQ=255)               ttATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAAt                                                                                                 >  2:518519/1‑139 (MQ=255)                      ttcACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGgc                                                                                          <  2:73740/139‑1 (MQ=255)                            ctgctgGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCgg                                                                                                          <  2:610037/117‑1 (MQ=255)                            ctgctgGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCgg                                                                                                          >  1:610037/1‑117 (MQ=255)                             tgctgGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGa                                                                                   >  1:405903/1‑139 (MQ=255)                                       cTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCt                                                                         >  2:84208/1‑139 (MQ=255)                                          cATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCg                                                                      <  1:84208/139‑1 (MQ=255)                                          cATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCg                                                                      <  2:872413/139‑1 (MQ=255)                                               tGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCggg                                                                                                         >  2:298302/1‑99 (MQ=255)                                               tGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCggg                                                                                                         <  1:298302/99‑1 (MQ=255)                                                 aTCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGAGTCCAGCCATCGCAGGCGGCCGGGGGGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTa                                                               >  1:521097/1‑139 (MQ=255)                                                    aaCTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAAcc                                                            >  1:88549/1‑139 (MQ=255)                                                           tACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGc                                                     >  1:818871/1‑139 (MQ=255)                                                           tACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGc                                                     <  2:537492/139‑1 (MQ=255)                                                           tACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGc                                                     <  2:821071/139‑1 (MQ=255)                                                           tACCGCTACTTTTGGTGGAGCAAGGATTACAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGc                                                     >  2:557197/1‑139 (MQ=255)                                                             ccGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGgcg                                                                                         >  1:583583/1‑101 (MQ=255)                                                             ccGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGgcg                                                                                         <  2:583583/101‑1 (MQ=255)                                                              cGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTAc                                                  >  2:400320/1‑139 (MQ=255)                                                                             aGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATTTTTGCACTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGgcg                                                                                         >  2:675384/1‑85 (MQ=255)                                                                             aGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGgcg                                                                                         <  1:675384/85‑1 (MQ=255)                                                                             aGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCa                                   >  1:353083/1‑139 (MQ=255)                                                                                aaGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGTTACTGATTTATAGCTGCATGt                                <  1:557197/139‑1 (MQ=255)                                                                                       ccAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTc                         <  2:540243/139‑1 (MQ=255)                                                                                         aGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCgc                       <  2:353083/139‑1 (MQ=255)                                                                                             aTCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCgctgct                   <  2:818871/139‑1 (MQ=255)                                                                                             aTCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCgctgct                   <  1:518519/139‑1 (MQ=255)                                                                                              tCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCGctgctg                  <  1:400320/139‑1 (MQ=255)                                                                                                              tGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCGCTGCTGGCGCTTGGAACGgggt  >  2:91988/1‑139 (MQ=255)                                                                                                                            cAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTc                                                                    >  2:459449/1‑59 (MQ=255)                                                                                                                            cAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTc                                                                    <  1:459449/59‑1 (MQ=255)                                                                                                                                 |                                                                                                                        CTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAAT‑GGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGT  >  NC_000913/376113‑376359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑