Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,215	(G)8→9	noncoding (72/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,207	1	.	G	100.0%	97.0 / NA	31	noncoding (80/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/13);  total (18/13)

AAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGA  >  NC_000913/4606071‑4606336                                                                                                                                          |                                                                                                                                  aaGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCTCCTTACTTTATACGATATCGCCTCATTTGGTCCGacg                                                                                                                                  >  1:100437/1‑137 (MQ=17)      aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGTCTCGTCTGGTGCGA‑ggggggg                                                                                                                            >  1:847232/1‑139 (MQ=255)        ttGGTGCGAGGGGGGGGACTTGATCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑ggggggggg                                                                                                                          <  1:633470/139‑2 (MQ=255)              cGAGGGGGGGGACTCGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTCTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGa                                                                                                                     >  2:613859/1‑139 (MQ=255)                                                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                              <  1:405117/125‑1 (MQ=255)                                                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                              >  2:405117/1‑125 (MQ=255)                                                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            <  2:891391/127‑1 (MQ=255)                                                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            >  1:891391/1‑127 (MQ=255)                                                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                                >  1:876922/1‑139 (MQ=255)                                                    cTAACACCTGAAGCTAGCGCGTCTACCAATCCCCCCACCTTCGCAAAGTCATCTTACTTTTTTTGATATCCCCTCGTTTGGTCCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                               <  1:662287/139‑1 (MQ=25)                                                       acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                            >  2:662287/1‑139 (MQ=255)                                                          ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                         >  1:506979/1‑139 (MQ=255)                                                               aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            >  1:257975/1‑115 (MQ=255)                                                               aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            <  2:257975/115‑1 (MQ=255)                                                                   aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                                >  2:980886/1‑139 (MQ=255)                                                                      gcgTCTACGAATTCCGCCACCTTCGCCCAGTCATCTTACTTTTTTTGAGATCCCCTCGTTTGGTGGGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                             <  1:613859/139‑1 (MQ=18)                                                                                atgccGCCAACTTCAAATAGACATCTTACTTTTTTTGATATCGACTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                   <  1:772925/136‑1 (MQ=17)                                                                                aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                   <  2:876922/139‑1 (MQ=255)                                                                                     gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                              >  1:432765/1‑139 (MQ=255)                                                                                      ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                             >  2:214125/1‑123 (MQ=255)                                                                                      ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                             <  1:214125/123‑1 (MQ=255)                                                                                       cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCGAGTACg                                            >  1:849361/1‑139 (MQ=21)                                                                                                    tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACg                               >  2:418522/1‑139 (MQ=255)                                                                                                       tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGa                                                                                                                     <  1:558811/50‑1 (MQ=38)                                                                                                       tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGa                                                                                                                     >  2:558811/1‑50 (MQ=38)                                                                                                       tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                          <  2:163910/125‑1 (MQ=255)                                                                                                       tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                          >  1:163910/1‑125 (MQ=255)                                                                                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                               <  1:832488/81‑1 (MQ=17)                                                                                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                               >  2:832488/1‑81 (MQ=17)                                                                                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                     >  2:641789/1‑139 (MQ=255)                                                                                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                     >  2:507394/1‑139 (MQ=255)                                                                                                                 tttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              <  1:179775/47‑1 (MQ=21)                                                                                                                 tttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              >  2:179775/1‑47 (MQ=21)                                                                                                                                 ttGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa  <  2:506979/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  AAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGA  >  NC_000913/4606071‑4606336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑