Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,767	A→G	intergenic (+136/‑56)	kefC → / → folA	potassium:proton antiporter/dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,767	0	A	G	100.0%	161.3 / NA	48	intergenic (+136/‑56)	kefC/folA	potassium:proton antiporter/dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/21);  total (27/21)

GAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAA  >  NC_000913/49636‑49890                                                                                                                                    |                                                                                                                            gagTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGTACCAGTTGGCGACGGt                                                                                                                      <  2:104678/139‑1 (MQ=255)  agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                     >  1:629467/1‑139 (MQ=255)  agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                     >  1:728363/1‑139 (MQ=255)  agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                     >  1:604079/1‑139 (MQ=255)      aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTTTTTCATTGTAGTGCGGCGAGTCCAGGTAGAGAGCGTGGACTCGCCAGCAGTATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACg                                                                                                                 >  2:643956/1‑139 (MQ=255)      aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCTTTGTAATGCGGCGAGTCCAGGGAGAGAGTGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGTGACGGTTTACg                                                                                                                 >  1:88826/1‑139 (MQ=255)      aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACg                                                                                                                 >  1:113744/1‑139 (MQ=255)       cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                <  2:728363/139‑1 (MQ=255)        gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                               <  2:629467/139‑1 (MQ=255)        gTAAATCACACTTTACAGCTAACTGTCTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGCAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                               <  1:643956/139‑1 (MQ=255)                 acTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGtata                                                                                                      <  1:377300/139‑1 (MQ=255)                  cTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAg                                                                                                     >  2:405611/1‑139 (MQ=255)                     tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                               >  2:430575/1‑126 (MQ=255)                     tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                               <  1:430575/126‑1 (MQ=255)                     tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTgg                                                                                                  >  1:664717/1‑139 (MQ=255)                             aCTGTTTGTTTTTGTTTCATTGTAATGCGTCGAGTCCATGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtt                                                                                          >  1:155536/1‑139 (MQ=255)                             aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtt                                                                                          >  1:89913/1‑139 (MQ=255)                               tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtttt                                                                                        >  2:666059/1‑139 (MQ=255)                                        ttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCgg                                                                               <  2:113744/139‑1 (MQ=255)                                          gtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                             <  2:604079/139‑1 (MQ=255)                                            ttCATTGTAATGCGGCGAGTCCAGGGAGAGAGTGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGTGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa                                                                           <  2:88826/139‑1 (MQ=255)                                            ttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa                                                                           <  1:666059/139‑1 (MQ=255)                                               aTTGTAATGCGACGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCTTGCTCGCACCAGTTGGCGACGGTTTACGCTTGACGATTAGCCGCGACCATTTTTTTTTTCGGTAAAtct                                                                        >  1:525523/1‑139 (MQ=255)                                                     aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGa                                                                  >  2:381686/1‑139 (MQ=255)                                                      tGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                             >  2:220630/1‑127 (MQ=255)                                                      tGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                             <  1:220630/127‑1 (MQ=255)                                                                       gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                <  2:89913/139‑1 (MQ=255)                                                                       gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                <  2:664717/139‑1 (MQ=255)                                                                        agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                >  1:691237/1‑74 (MQ=255)                                                                        agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                <  2:691237/74‑1 (MQ=255)                                                                        agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt                                               >  1:509087/1‑139 (MQ=255)                                                                        agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACACCATCCTCGCACCAGTTGGCGACTGTTTACGCTTTACGTATAGGGGCGACAATTTTTTTTATCGTGAAATCTCAATGATCAGCCTGATTGCGGCGtt                                               >  1:289145/1‑139 (MQ=255)                                                                         gagCGTGGTCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTa                                              <  2:991229/139‑1 (MQ=255)                                                                         gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTa                                              >  2:942057/1‑139 (MQ=255)                                                                            cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCg                                           >  1:983818/1‑139 (MQ=255)                                                                                     gCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATcgc                                  >  2:571140/1‑139 (MQ=255)                                                                                         gcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa                              <  1:571140/139‑1 (MQ=255)                                                                                               tataAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCa                        >  2:420381/1‑139 (MQ=255)                                                                                                        ttCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACg               >  2:588842/1‑139 (MQ=255)                                                                                                         tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGc              >  2:18573/1‑139 (MQ=255)                                                                                                          ccTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc             <  1:381686/139‑1 (MQ=255)                                                                                                            tCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCAt           <  2:919693/139‑1 (MQ=255)                                                                                                                catcatCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg       <  2:509087/139‑1 (MQ=255)                                                                                                                   catcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTgg    <  2:155536/139‑1 (MQ=255)                                                                                                                     tcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGaa  >  2:558292/1‑139 (MQ=255)                                                                                                                     tcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGaa  >  2:969803/1‑139 (MQ=255)                                                                                                                        tCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg                                                    <  2:561382/86‑1 (MQ=255)                                                                                                                        tCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg                                                    >  1:561382/1‑86 (MQ=255)                                                                                                                                    |                                                                                                                            GAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAA  >  NC_000913/49636‑49890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑