Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	518,721	C→A	M143I (ATG→ATT)	ybbO ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	518,721	0	C	A	100.0%	121.9 / NA	38	M143I (ATG→ATT)	ybbO	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/17);  total (21/17)

ATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATA  >  NC_000913/518605‑518852                                                                                                                     |                                                                                                                                    aTTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGt                                                                                                               >  2:41818/1‑139 (MQ=255) aTTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGt                                                                                                               >  2:314067/1‑139 (MQ=255)      gCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGt                                                                                                          <  1:798887/139‑1 (MQ=255)                    ccATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTaa                                                                                            <  1:773688/139‑1 (MQ=255)                          gcAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGgcg                                                                                      <  1:197769/139‑1 (MQ=255)                              tGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATg                                                                                  >  2:785447/1‑139 (MQ=255)                              tGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATg                                                                                  >  2:483979/1‑139 (MQ=255)                                   cTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAg                                                                             >  1:670129/1‑139 (MQ=255)                                        cACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGgt                                                                        >  1:673454/1‑139 (MQ=255)                                          cGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATa                                                                                                                            <  1:162738/85‑1 (MQ=255)                                          cGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATa                                                                                                                            >  2:162738/1‑85 (MQ=255)                                          cGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCAc                                                                                                                  >  2:644764/1‑95 (MQ=255)                                          cGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCAc                                                                                                                  <  1:644764/95‑1 (MQ=255)                                                                gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATcaca                                                                                                                               <  2:965533/60‑1 (MQ=255)                                                                gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATcaca                                                                                                                               >  1:965533/1‑60 (MQ=255)                                                                gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATcg                                                                                                   >  1:207959/1‑88 (MQ=255)                                                                gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATcg                                                                                                   <  2:207959/88‑1 (MQ=255)                                                                  cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAAc                                              >  2:374552/1‑139 (MQ=255)                                                                    cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAAt                                                                                                                             <  2:416713/58‑1 (MQ=255)                                                                    cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAAt                                                                                                                             >  1:416713/1‑58 (MQ=255)                                                                     gTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActgc                                           <  2:670129/139‑1 (MQ=255)                                                                     gTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActgc                                           <  1:41818/139‑1 (MQ=255)                                                                             cACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGCCGGAAAACTGCTGTTCCAt                                   <  2:673454/139‑1 (MQ=255)                                                                               cGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCt                                 >  2:857845/1‑139 (MQ=255)                                                                                         tGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGTCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCt                       <  1:785447/139‑1 (MQ=255)                                                                                           gagaTTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCg                                                                                                                <  2:199230/48‑1 (MQ=255)                                                                                           gagaTTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCg                                                                                                                >  1:199230/1‑48 (MQ=255)                                                                                           gagaTTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGa                     >  1:582161/1‑139 (MQ=255)                                                                                             gaTTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGTAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGAtg                   <  1:314067/139‑1 (MQ=255)                                                                                                    cccATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGaaa            <  1:374552/139‑1 (MQ=255)                                                                                                        tCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTgcg                                                                   >  2:311349/1‑80 (MQ=255)                                                                                                        tCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTgcg                                                                   <  1:311349/80‑1 (MQ=255)                                                                                                        tCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGaaa            <  2:576503/135‑1 (MQ=255)                                                                                                        tCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGaaa            >  1:576503/1‑135 (MQ=255)                                                                                                        tCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAgggg        >  1:847004/1‑139 (MQ=255)                                                                                                        tCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAgggg        >  1:615438/1‑139 (MQ=255)                                                                                                              atgatgTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATa  >  2:862053/1‑139 (MQ=255)                                                                                                              atgatgTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATa  >  2:941801/1‑139 (MQ=255)                                                                                                                     |                                                                                                                                    ATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATA  >  NC_000913/518605‑518852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑