Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	586,280	T→C	N210S (AAC→AGC)	envY ←	porin thermoregulatory transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	586,280	0	T	C	100.0%	191.3 / NA	53	N210S (AAC→AGC)	envY	porin thermoregulatory transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/25);  total (28/25)

CTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAATTCGCA  >  NC_000913/586145‑586415                                                                                                                                        |                                                                                                                                        cTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTAt                                                                                                                                      <  1:45371/139‑1 (MQ=255)        acaTCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCaa                                                                                                                               >  1:963586/1‑139 (MQ=255)         caTCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              <  1:350650/139‑1 (MQ=255)         caTCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              <  1:889284/139‑1 (MQ=255)                      cGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCTGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACgg                                                                                                                 >  2:2847/1‑139 (MQ=255)                          gTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTa                                                                                                             <  2:963586/139‑1 (MQ=255)                                cGAGATAATTCAACGGTGTCAGGCTGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCAt                                                                                                       <  1:2847/139‑1 (MQ=255)                                    aTAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGa                                                                                                   <  2:876894/139‑1 (MQ=255)                                    aTAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGa                                                                                                   <  1:497005/139‑1 (MQ=255)                                                tgtCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTTCGCCACCTGAGTGATATTTTTGCTAt                                                                                                                                      >  2:260202/1‑92 (MQ=255)                                                tgtCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTAt                                                                                                                                      <  1:577745/92‑1 (MQ=255)                                                tgtCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTAt                                                                                                                                      <  1:260202/92‑1 (MQ=255)                                                tgtCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTAt                                                                                                                                      >  2:577745/1‑92 (MQ=255)                                                  tCAGGCCGTAAAACGCCTTAAATACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATcc                                                                                                                                    <  1:587637/92‑1 (MQ=255)                                                  tCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATcc                                                                                                                                    >  2:587637/1‑92 (MQ=255)                                                  tCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACAt                                                                                                                         >  1:526564/1‑103 (MQ=255)                                                  tCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACAt                                                                                                                         <  2:526564/103‑1 (MQ=255)                                                          tAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCtat                                                                             >  1:192525/1‑139 (MQ=255)                                                          tAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCt                                                                               >  1:400739/1‑137 (MQ=255)                                                          tAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCt                                                                               <  2:400739/137‑1 (MQ=255)                                                          tAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATAAGACACTCTGTGACAATCTGGCtat                                                                             >  2:520241/1‑139 (MQ=255)                                                                            gagaTAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTa                                                                                                                                       >  1:396315/1‑63 (MQ=255)                                                                            gagaTAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTa                                                                                                                                       <  2:396315/63‑1 (MQ=255)                                                                                         acgTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGc                                                                                                         <  2:482603/80‑1 (MQ=255)                                                                                         acgTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGc                                                                                                         >  1:482603/1‑80 (MQ=255)                                                                                         acgTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCttttt                                              >  2:145889/1‑139 (MQ=255)                                                                                          cgTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGc                                                                                                                >  1:39308/1‑72 (MQ=255)                                                                                          cgTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGc                                                                                                                <  2:39308/72‑1 (MQ=255)                                                                                            tgctgcTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              >  2:788907/1‑56 (MQ=255)                                                                                            tgctgcTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              <  1:788907/56‑1 (MQ=255)                                                                                              ctgctATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCa                                         >  1:662208/1‑139 (MQ=255)                                                                                               tgctATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAAc                                                                                                           <  2:87684/72‑1 (MQ=255)                                                                                               tgctATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAAc                                                                                                           >  1:87684/1‑72 (MQ=255)                                                                                                   ataGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTg                                    >  2:904974/1‑139 (MQ=255)                                                                                                           aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              <  2:353698/41‑1 (MQ=255)                                                                                                           aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              <  1:738620/41‑1 (MQ=255)                                                                                                           aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              >  2:738620/1‑41 (MQ=255)                                                                                                           aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              >  1:353698/1‑41 (MQ=255)                                                                                                           aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCt                                     >  2:199563/1‑130 (MQ=255)                                                                                                           aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCt                                     <  1:199563/130‑1 (MQ=255)                                                                                                           aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTaa                            >  2:810113/1‑139 (MQ=255)                                                                                                           aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTaa                            >  2:492994/1‑139 (MQ=255)                                                                                                                tGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATa                       >  2:981903/1‑139 (MQ=255)                                                                                                                    ccACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCtgt                                                                                           >  2:297571/1‑67 (MQ=255)                                                                                                                    ccACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCtgt                                                                                           <  1:297571/67‑1 (MQ=255)                                                                                                                       ccTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACt                <  2:192525/139‑1 (MQ=255)                                                                                                                       ccTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACt                >  1:610641/1‑139 (MQ=255)                                                                                                                               atatTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCt                                     <  2:342524/110‑1 (MQ=255)                                                                                                                               atatTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCt                                     >  1:342524/1‑110 (MQ=255)                                                                                                                                 atTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGa                                                                                                   <  1:620143/46‑1 (MQ=255)                                                                                                                                 atTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAAtt      >  2:509260/1‑139 (MQ=255)                                                                                                                                 atTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCAAACGa                                                                                                   >  2:620143/1‑46 (MQ=255)                                                                                                                                     ttGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAATTCGCa  <  2:610641/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAATTCGCA  >  NC_000913/586145‑586415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑