Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	607,988	G→A	*51* (TAG→TAA)	hokE →	toxic polypeptide, small

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	607,988	0	G	A	100.0%	195.2 / NA	61	*51* (TAG→TAA)	hokE	toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/33);  total (28/33)

TTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAA  >  NC_000913/607855‑608121                                                                                                                                      |                                                                                                                                      ttGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGtt                                                                                                                                  <  2:840544/139‑1 (MQ=255)  tGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTtg                                                                                                                                 >  1:272514/1‑139 (MQ=255)   gCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTtgt                                                                                                                                >  2:467802/1‑139 (MQ=255)      gCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                                             >  1:403261/1‑139 (MQ=255)      gCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                                             <  1:653911/139‑1 (MQ=255)                     tgtTTAATGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATa                                                                                                              >  1:661962/1‑139 (MQ=255)                     tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATa                                                                                                              >  1:174217/1‑139 (MQ=255)                       tttAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAg                                                                                                            >  2:53980/1‑139 (MQ=255)                                  tGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTaa                                                                                                 <  1:912623/139‑1 (MQ=255)                                     gATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                              <  1:384769/139‑1 (MQ=255)                                      aTTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGt                                                                                             <  2:661962/139‑1 (MQ=255)                                         tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                                             <  2:647304/104‑1 (MQ=255)                                         tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                                             >  1:647304/1‑104 (MQ=255)                                           cGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTgg                                                                                        >  1:111853/1‑139 (MQ=255)                                                cTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                        <  2:219575/118‑1 (MQ=255)                                                cTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                        >  1:219575/1‑118 (MQ=255)                                                cTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                                  <  2:410431/124‑1 (MQ=255)                                                cTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                                  >  1:410431/1‑124 (MQ=255)                                                 tGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                        >  1:831113/1‑117 (MQ=255)                                                 tGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                        <  2:831113/117‑1 (MQ=255)                                                  ggTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACg                                                                                 <  1:467802/139‑1 (MQ=255)                                                   gTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGc                                                                                <  2:272514/139‑1 (MQ=255)                                                    tCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGcc                                                                               <  2:174217/139‑1 (MQ=255)                                                    tCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGcc                                                                               <  2:912326/139‑1 (MQ=255)                                                        agaCTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCggg                                                                                                                           <  1:612896/91‑1 (MQ=255)                                                        agaCTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCggg                                                                                                                           >  2:612896/1‑91 (MQ=255)                                                        agaCTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGat                                                                           <  2:715886/139‑1 (MQ=255)                                                                          tACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGAt                                                         >  2:515864/1‑139 (MQ=255)                                                                            cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                        >  2:985385/1‑90 (MQ=255)                                                                            cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                        <  1:985385/90‑1 (MQ=255)                                                                            cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                              <  2:239433/100‑1 (MQ=255)                                                                            cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                              >  1:239433/1‑100 (MQ=255)                                                                             ggTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGaa                                                      <  2:229984/139‑1 (MQ=255)                                                                                 aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAc                                                  >  2:561132/1‑139 (MQ=255)                                                                                 aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCAGATGAATTAc                                                  >  2:179003/1‑139 (MQ=255)                                                                                  aGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                 <  2:752697/139‑1 (MQ=255)                                                                                  aGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                 >  1:796401/1‑139 (MQ=255)                                                                                  aGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                 <  2:796401/139‑1 (MQ=255)                                                                                  aGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                 <  2:403261/139‑1 (MQ=255)                                                                                           atatTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                              <  2:544191/85‑1 (MQ=255)                                                                                           atatTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                              >  1:544191/1‑85 (MQ=255)                                                                                              ttGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGcc                                                                               >  2:148569/1‑97 (MQ=255)                                                                                              ttGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGcc                                                                               <  1:148569/97‑1 (MQ=255)                                                                                                   tttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCa                                >  1:953165/1‑139 (MQ=255)                                                                                                      aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGt                                                                                                                       <  2:129563/49‑1 (MQ=255)                                                                                                      aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGt                                                                                                                       >  1:129563/1‑49 (MQ=255)                                                                                                      aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCa                             >  1:863042/1‑139 (MQ=255)                                                                                                      aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCa                             >  1:761319/1‑139 (MQ=255)                                                                                                      aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCa                             >  2:903020/1‑139 (MQ=255)                                                                                                            gTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTg                       <  2:311284/139‑1 (MQ=255)                                                                                                             ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                              >  1:946731/1‑67 (MQ=255)                                                                                                             ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                              <  2:946731/67‑1 (MQ=255)                                                                                                             ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAt                                         >  1:983080/1‑120 (MQ=255)                                                                                                             ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAt                                         <  2:983080/120‑1 (MQ=255)                                                                                                             ttCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTgg                      <  1:53980/139‑1 (MQ=255)                                                                                                              tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                     <  2:111853/139‑1 (MQ=255)                                                                                                              tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                     <  1:903020/139‑1 (MQ=255)                                                                                                                       cGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                          >  2:192188/1‑125 (MQ=255)                                                                                                                       cGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                          <  1:192188/125‑1 (MQ=255)                                                                                                                       cGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTgg            <  2:647452/139‑1 (MQ=255)                                                                                                                                 aagTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCgaa  <  1:561132/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAA  >  NC_000913/607855‑608121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑