Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,153	C→A	E42D (GAG→GAT)	lipB ←	octanoyltransferase, octanoyl‑[ACP]:protein N‑octanoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,153	0	C	A	100.0%	163.6 / NA	51	E42D (GAG→GAT)	lipB	octanoyltransferase, octanoyl‑[ACP]:protein N‑octanoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/27);  total (24/27)

ACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCA  >  NC_000913/662019‑662285                                                                                                                                       |                                                                                                                                     acatacatCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGa                                                                                                                                  <  1:222850/139‑1 (MQ=255)  catacatCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGAc                                                                                                                                 <  1:585988/139‑1 (MQ=255)        tCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGAccagcca                                                                                                                           <  2:913954/139‑1 (MQ=255)          aCCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGa                                                                                                                         >  2:244901/1‑139 (MQ=255)          aCCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGa                                                                                                                         >  1:732423/1‑139 (MQ=255)          aCCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGCGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGa                                                                                                                         >  1:49458/1‑139 (MQ=255)           ccTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGAt                                                                                                                        >  2:540092/1‑139 (MQ=255)                 gCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  >  1:569732/1‑139 (MQ=255)                 gCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  <  1:928657/139‑1 (MQ=255)                 gCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  <  1:540092/139‑1 (MQ=255)                  ccccGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatca                                                                                                                 <  1:353636/139‑1 (MQ=255)                    ccGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAg                                                                                                               >  2:865276/1‑139 (MQ=255)                      ggCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAggg                                                                                                             <  2:389421/139‑1 (MQ=255)                       gCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGt                                                                                                            <  1:37980/139‑1 (MQ=255)                          cGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACt                                                                                                         <  2:569732/139‑1 (MQ=255)                            tGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTat                                                                                                       >  1:976363/1‑139 (MQ=255)                             gATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGAccagc                                                                                                                             <  1:965469/116‑1 (MQ=255)                             gATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGAccagc                                                                                                                             >  2:965469/1‑116 (MQ=255)                                aaGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTatcatc                                                                                                   <  1:865276/139‑1 (MQ=255)                                 aGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATcg                                                                                                  >  1:554553/1‑139 (MQ=255)                                      ccTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGAcc                                                                                                                                >  2:799213/1‑104 (MQ=255)                                      ccTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGAcc                                                                                                                                <  1:799213/104‑1 (MQ=255)                                      ccTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGt                                                                                             >  1:820494/1‑139 (MQ=255)                                            cACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGt                                                                                       <  2:976363/139‑1 (MQ=255)                                            cACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCGTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGt                                                                                       <  2:49458/139‑1 (MQ=255)                                             aCCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGGCTGACCATGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAggg                                                                                                             <  2:569236/116‑1 (MQ=255)                                             aCCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAggg                                                                                                             >  1:569236/1‑116 (MQ=255)                                                    tCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCAt                                                                               >  2:248132/1‑139 (MQ=255)                                                      gCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGc                                                                             <  2:780351/139‑1 (MQ=255)                                                       ctctGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                            <  2:732423/139‑1 (MQ=255)                                                       ctctGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                            <  1:248132/139‑1 (MQ=255)                                                        tctGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCAt                                                                           <  2:282999/139‑1 (MQ=255)                                                                aCCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTggg                                                                   >  1:245583/1‑139 (MQ=255)                                                                    gAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGAt                                                               <  1:164740/139‑1 (MQ=255)                                                                    gAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATAAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGAt                                                               >  1:675516/1‑139 (MQ=255)                                                                            cccGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGt                                                       >  2:399819/1‑139 (MQ=255)                                                                            cccGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGt                                                       >  1:233182/1‑139 (MQ=255)                                                                             ccGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTa                                                      <  1:907224/139‑1 (MQ=255)                                                                               ggCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTg                                                                                      <  1:130764/105‑1 (MQ=255)                                                                               ggCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTg                                                                                      >  2:130764/1‑105 (MQ=255)                                                                                 cATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGc                                                  >  2:246866/1‑139 (MQ=255)                                                                                                                  ttGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATcc                 <  1:246866/139‑1 (MQ=255)                                                                                                                     ggTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTAt                                                                                           <  1:337923/62‑1 (MQ=255)                                                                                                                     ggTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTAt                                                                                           >  2:337923/1‑62 (MQ=255)                                                                                                                     ggTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGa              >  1:781356/1‑139 (MQ=255)                                                                                                                          aTACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACaa         >  1:677608/1‑139 (MQ=255)                                                                                                                           tACCGGATAGTGATCGACCAGACAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGa              <  1:948802/133‑1 (MQ=255)                                                                                                                           tACCGGATAGTGATCGACCAGACAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGa              >  2:948802/1‑133 (MQ=255)                                                                                                                                gATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGc   >  2:955074/1‑139 (MQ=255)                                                                                                                                 aTAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCa  <  2:677608/139‑1 (MQ=255)                                                                                                                                 aTAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCa  <  2:245583/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     ACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCA  >  NC_000913/662019‑662285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑