| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 775,120 | G→A | A123A (GCG→GCA) | ybgC → | acyl‑CoA thioester hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 775,120 | 0 | G | A | 100.0% | 189.8 / NA | 58 | A123A (GCG→GCA) | ybgC | acyl‑CoA thioester hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/30); total (28/30) | |||||||||||
GACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGC > NC_000913/774988‑775250 | gACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc > 2:25851/1‑139 (MQ=255)gACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc < 1:25851/139‑1 (MQ=255)gACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc > 1:928578/1‑139 (MQ=255)gACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTccc < 1:45495/139‑1 (MQ=255) cTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCaa < 1:980143/139‑1 (MQ=255) cATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGt > 1:945699/1‑139 (MQ=255) aTCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTc > 1:229064/1‑139 (MQ=255) aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgcg > 2:360159/1‑139 (MQ=255) agCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCGAACTCTATTGTCGCGGa > 2:622254/2‑139 (MQ=255) tGGCACCTCTTTGGTTTTCACGCATCGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGttt < 2:564376/139‑1 (MQ=255) tGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGttt > 2:963036/1‑139 (MQ=255) ctctTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAg > 2:507313/1‑139 (MQ=255) gTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactga < 2:928578/139‑1 (MQ=255) gTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactga < 1:963036/139‑1 (MQ=255) aCGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa < 1:507313/139‑1 (MQ=255) aCGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa < 2:945699/139‑1 (MQ=255) aTTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGa > 1:388020/1‑139 (MQ=255) gTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGAttt > 2:882755/1‑139 (MQ=255) cAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAACTCTATTGTCCCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGt < 1:622254/139‑1 (MQ=255) aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgcg < 2:21091/98‑1 (MQ=255) aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTcgcg > 1:21091/1‑98 (MQ=255) aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTc > 2:853533/1‑139 (MQ=255) aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTc > 2:83088/1‑139 (MQ=255) gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTg > 1:847348/1‑134 (MQ=255) gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTg < 2:847348/134‑1 (MQ=255) gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCt > 2:884543/1‑139 (MQ=255) gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCt > 2:584019/1‑139 (MQ=255) gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCt > 1:189925/1‑139 (MQ=255) gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCt < 2:229064/139‑1 (MQ=255) gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCt < 2:189925/139‑1 (MQ=255) ccGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTg < 1:360159/139‑1 (MQ=255) ttGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt < 1:882755/139‑1 (MQ=255) ttGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGACTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt < 1:584019/139‑1 (MQ=255) tGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCtt < 1:814906/139‑1 (MQ=255) cTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCt > 1:133899/1‑139 (MQ=255) aatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa < 1:442724/103‑1 (MQ=255) aatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa > 2:442724/1‑103 (MQ=255) aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa > 1:123670/1‑98 (MQ=255) aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa < 2:123670/98‑1 (MQ=255) agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt < 2:908724/123‑1 (MQ=255) agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt > 1:908724/1‑123 (MQ=255) gTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAg < 1:157946/75‑1 (MQ=255) gTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAg > 2:157946/1‑75 (MQ=255) ggTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGa < 2:955052/85‑1 (MQ=255) ggTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGa > 1:955052/1‑85 (MQ=255) ttGCGTTGACCCACTCAAAATGTAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCt < 2:641878/86‑1 (MQ=255) ttGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAg < 2:818122/64‑1 (MQ=255) ttGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAg > 1:818122/1‑64 (MQ=255) ttGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCt > 1:641878/1‑86 (MQ=255) tGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCt > 2:586433/1‑97 (MQ=255) tGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTGGATTTGGTCCt < 1:586433/97‑1 (MQ=255) ttGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt < 2:751000/105‑1 (MQ=255) ttGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt > 1:751000/1‑105 (MQ=255) aCTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCaa < 1:83088/139‑1 (MQ=255) tGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCt < 2:269578/139‑1 (MQ=255) aGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTgg > 1:110881/1‑139 (MQ=255) ccTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc < 2:133899/139‑1 (MQ=255) ccTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc < 1:853533/139‑1 (MQ=255) | GACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGC > NC_000913/774988‑775250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |