Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	776,397	C→T	A19V (GCA→GTA)	tolA →	membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	776,397	0	C	T	100.0%	112.1 / NA	36	A19V (GCA→GTA)	tolA	membrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/22);  total (14/22)

GCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGG  >  NC_000913/776265‑776519                                                                                                                                     |                                                                                                                           gCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGtgct                                                                                                                      <  1:730138/139‑1 (MQ=255)     ccTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCAt                                                                                                                  <  1:215270/139‑1 (MQ=255)                   tGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgc                                                                                                    >  2:447600/1‑139 (MQ=255)                   tGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgc                                                                                                    >  2:597711/1‑139 (MQ=255)                   tGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgc                                                                                                    >  1:424172/1‑139 (MQ=255)                     cGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGtgc                                                                                                                       <  1:777854/118‑1 (MQ=255)                     cGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGtgc                                                                                                                       >  2:777854/1‑118 (MQ=255)                     cGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcgg                                                                                                  <  1:875998/139‑1 (MQ=255)                       tttCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCACAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                <  1:638661/139‑1 (MQ=255)                         tCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCt                                                                                              <  2:961485/139‑1 (MQ=255)                            cttgcttgAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGAt                                                                                           <  1:597711/139‑1 (MQ=255)                             ttgcttgAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATc                                                                                          >  1:875296/1‑139 (MQ=255)                                          agCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGttcgttcg                                                                             >  1:552282/1‑139 (MQ=255)                                            cGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                           <  2:748489/139‑1 (MQ=255)                                            cGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                           <  2:525541/139‑1 (MQ=255)                                                         aaCAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcgg                                                                                                  >  2:932143/1‑103 (MQ=255)                                                         aaCAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcgg                                                                                                  <  1:932143/103‑1 (MQ=255)                                                           cAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCt                                                            >  2:869506/1‑139 (MQ=255)                                                                      aaCCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAgg                                                 <  2:424172/139‑1 (MQ=255)                                                                                 tCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGtt                                      <  1:447600/139‑1 (MQ=255)                                                                                   aaaGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCAt                                                                                                                  >  1:302981/1‑61 (MQ=255)                                                                                   aaaGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCAt                                                                                                                  <  2:302981/61‑1 (MQ=255)                                                                                           ccGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAgttcgttc                                                                              <  2:643521/89‑1 (MQ=255)                                                                                           ccGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAgttcgttc                                                                              >  1:643521/1‑89 (MQ=255)                                                                                           ccGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                           >  1:812876/1‑92 (MQ=255)                                                                                           ccGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                           <  2:812876/92‑1 (MQ=255)                                                                                           ccGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGa                            <  1:869506/139‑1 (MQ=255)                                                                                                     cGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATg                  <  2:853847/139‑1 (MQ=255)                                                                                                       aCAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGt                <  2:552282/139‑1 (MQ=255)                                                                                                           gCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                >  1:992703/1‑55 (MQ=255)                                                                                                           gCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                <  2:992703/55‑1 (MQ=255)                                                                                                                    ggCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCg   >  2:76602/1‑139 (MQ=255)                                                                                                                     gCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCgg  <  2:875296/139‑1 (MQ=255)                                                                                                                     gCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCgg  <  1:555110/139‑1 (MQ=255)                                                                                                                                ttCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                           >  2:694640/1‑55 (MQ=255)                                                                                                                                ttCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                           <  1:694640/55‑1 (MQ=255)                                                                                                                                     |                                                                                                                           GCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGG  >  NC_000913/776265‑776519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑