Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,115	G→A	*255* (TAG→TAA)	yabI →	DedA family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,115	0	G	A	100.0%	129.0 / NA	41	*255* (TAG→TAA)	yabI	DedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/22);  total (19/22)

TATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTA  >  NC_000913/71981‑72238                                                                                                                                       |                                                                                                                            tattTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAgaga                                                                                                                         <  1:830959/139‑1 (MQ=255)     tGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGt                                                                                                                     >  1:650831/1‑139 (MQ=255)         ccGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTtg                                                                                                                 <  1:131685/139‑1 (MQ=255)           gcgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTtgtg                                                                                                               >  2:800495/1‑139 (MQ=255)                    tgttgtGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGcc                                                                                                      <  1:823645/139‑1 (MQ=255)                               aCGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGc                                                                                           >  1:853434/1‑139 (MQ=255)                                cGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCt                                                                                          >  1:342841/1‑139 (MQ=255)                                   cGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                       >  1:210929/1‑139 (MQ=255)                                     ttGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                       <  1:239134/137‑1 (MQ=255)                                     ttGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                       >  2:239134/1‑137 (MQ=255)                                      tGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCcgc                                                                                    <  2:497988/139‑1 (MQ=255)                                             tGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                             >  2:258087/1‑139 (MQ=255)                                             tGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                             <  1:646921/139‑1 (MQ=255)                                               ccATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                           >  2:973185/1‑139 (MQ=255)                                                 aTCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                         <  2:650831/139‑1 (MQ=255)                                                              cgcTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCa                                                            <  1:258087/139‑1 (MQ=255)                                                              cgcTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCATACAAAACGCa                                                            <  2:342841/139‑1 (MQ=255)                                                               gcTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTTAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCAc                                                           <  2:535746/139‑1 (MQ=255)                                                                         ttAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAgg                                                 <  1:800495/139‑1 (MQ=255)                                                                                gCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGc                                                             >  1:999757/1‑120 (MQ=255)                                                                                gCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGc                                                             <  2:999757/120‑1 (MQ=255)                                                                                       gTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAgg                                                 >  1:302309/1‑125 (MQ=255)                                                                                       gTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAgg                                                 <  2:302309/125‑1 (MQ=255)                                                                                           aTGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcg                               <  2:210929/139‑1 (MQ=255)                                                                                           aTGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcg                               <  1:388488/139‑1 (MQ=255)                                                                                                  tgtATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATc                        >  1:844778/1‑139 (MQ=255)                                                                                                      tatCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGAc                    >  2:105969/1‑139 (MQ=255)                                                                                                           atatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAg                                                  <  2:444960/104‑1 (MQ=255)                                                                                                           atatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAg                                                  >  1:444960/1‑104 (MQ=255)                                                                                                            tatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                       >  1:645769/1‑66 (MQ=255)                                                                                                            tatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                       <  2:645769/66‑1 (MQ=255)                                                                                                              ttttGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACg                                                              <  1:829826/89‑1 (MQ=255)                                                                                                              ttttGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACg                                                              >  2:829826/1‑89 (MQ=255)                                                                                                                 tGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCa                                                            <  2:189118/88‑1 (MQ=255)                                                                                                                 tGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCa                                                            >  1:189118/1‑88 (MQ=255)                                                                                                                     tAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAAccc     >  1:91317/1‑139 (MQ=255)                                                                                                                        aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCcgcg                                                                                   <  2:278021/58‑1 (MQ=255)                                                                                                                        aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCcgcg                                                                                   >  1:278021/1‑58 (MQ=255)                                                                                                                        aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa  <  1:16705/139‑1 (MQ=255)                                                                                                                             ttGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                       >  1:548295/1‑49 (MQ=255)                                                                                                                             ttGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                       <  2:548295/49‑1 (MQ=255)                                                                                                                                       |                                                                                                                            TATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTA  >  NC_000913/71981‑72238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑