Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	892,869	G→A	*301* (TAG→TAA)	rimK →	ribosomal protein S6 modification protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	892,869	0	G	A	100.0%	200.0 / NA	63	*301* (TAG→TAA)	rimK	ribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/32);  total (31/32)

TGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAAT  >  NC_000913/892736‑892999                                                                                                                                      |                                                                                                                                   tGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCa                                                                                                                               >  1:801880/1‑139 (MQ=255)   gTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             <  2:946369/139‑1 (MQ=255)   gTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             <  2:462319/139‑1 (MQ=255)   gTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             <  2:742662/139‑1 (MQ=255)   gTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             <  1:187016/139‑1 (MQ=255)          aGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                      >  1:435016/1‑139 (MQ=255)           ggTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                              <  1:198655/130‑1 (MQ=255)           ggTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                              >  2:198655/1‑130 (MQ=255)              gAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGa                                                                                                                  >  2:701703/1‑139 (MQ=255)                         cGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATcaca                                                                                                                         >  2:71532/1‑121 (MQ=255)                         cGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATcaca                                                                                                                         <  1:71532/121‑1 (MQ=255)                             gCTGGAAGGAATAGAAAAAACCACCGGTATCGACGTCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           >  2:60920/1‑115 (MQ=255)                             gCTGGAAGGAATAGAAAAAACCACCGGTATCGACGTCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           <  1:60920/115‑1 (MQ=255)                                        tAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCg                                                                                        <  2:435016/139‑1 (MQ=255)                                        tAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACAGATCATGGTTTTGCCTGCGCTTTTTGCg                                                                                        >  2:278994/1‑139 (MQ=255)                                               aaCCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCtg                                                                                 >  1:190112/1‑139 (MQ=255)                                                accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             >  2:17450/1‑94 (MQ=255)                                                accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             <  1:17450/94‑1 (MQ=255)                                                accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCtgt                                                                                >  2:76809/1‑139 (MQ=255)                                                   accGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                             <  2:190112/139‑1 (MQ=255)                                                     cGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           <  1:299770/91‑1 (MQ=255)                                                     cGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           >  2:299770/1‑91 (MQ=255)                                                                 cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTGCTGATCATGGTTTTGCCTGCGCtt                                                                                              <  1:684325/108‑1 (MQ=255)                                                                 cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCtt                                                                                              >  2:684325/1‑108 (MQ=255)                                                                 cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGa                                                               >  2:865709/1‑139 (MQ=255)                                                                 cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGa                                                               >  2:63734/1‑139 (MQ=255)                                                                  gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGaa                                                              <  1:14364/139‑1 (MQ=255)                                                                   cgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           >  1:570171/1‑77 (MQ=255)                                                                   cgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           <  2:570171/77‑1 (MQ=255)                                                                         aaaTGATCCGCTGGATCGAGCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGtt                                                       >  2:673876/1‑139 (MQ=255)                                                                            tGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTa                                                    <  1:798682/139‑1 (MQ=255)                                                                               tCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           <  1:902306/65‑1 (MQ=255)                                                                               tCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           >  2:902306/1‑65 (MQ=255)                                                                                ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           <  1:91856/64‑1 (MQ=255)                                                                                ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           >  2:91856/1‑64 (MQ=255)                                                                                ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                             <  1:656196/110‑1 (MQ=255)                                                                                ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACaaa                                                >  1:321198/1‑139 (MQ=255)                                                                                ccGCTGGAGCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                             >  2:656196/1‑110 (MQ=255)                                                                                      gATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTTATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATg                                          <  1:949821/139‑1 (MQ=255)                                                                                      gATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATg                                          <  1:701703/139‑1 (MQ=255)                                                                                           aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATc                                     >  2:236823/1‑139 (MQ=255)                                                                                              gCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACCTTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAGAATTATGACATCGCt                                  <  1:673876/139‑1 (MQ=255)                                                                                                 aCGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                               <  2:570461/139‑1 (MQ=255)                                                                                                  cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                                <  1:279196/41‑1 (MQ=255)                                                                                                  cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                                >  2:279196/1‑41 (MQ=255)                                                                                                            gAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAGAATTATGACATCGCTGGTCGTTCCTGGTc                    <  2:290771/139‑1 (MQ=255)                                                                                                             aaTATTGTCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                      >  2:970267/1‑40 (MQ=255)                                                                                                             aaTATTGTCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                      <  1:970267/40‑1 (MQ=37)                                                                                                             aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCt                   >  2:367707/1‑139 (MQ=255)                                                                                                                  tGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATa              >  2:37005/1‑139 (MQ=255)                                                                                                                   gCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATAc             <  1:278994/139‑1 (MQ=255)                                                                                                                   gCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATAc             <  1:236823/139‑1 (MQ=255)                                                                                                                   gCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCCTTTTATCGAAAGAGGTTGTACAACATTATGACATCGCTGGTCGTTCCTGGTCTGGATAc             >  2:824567/1‑139 (MQ=255)                                                                                                                       gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTa                                                                                      <  1:803922/62‑1 (MQ=255)                                                                                                                       gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTa                                                                                      >  2:803922/1‑62 (MQ=255)                                                                                                                       gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAgg                                                         >  2:498540/1‑91 (MQ=255)                                                                                                                       gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAgg                                                         <  1:498540/91‑1 (MQ=255)                                                                                                                       gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTg         >  2:466293/1‑139 (MQ=255)                                                                                                                         aaaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTa                                                    <  1:22819/94‑1 (MQ=255)                                                                                                                         aaaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTa                                                    >  2:22819/1‑94 (MQ=255)                                                                                                                         aaaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTcc                         >  1:564974/1‑121 (MQ=255)                                                                                                                         aaaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTcc                         <  2:564974/121‑1 (MQ=255)                                                                                                                             gggTGGTTTATCGCACTCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTTCTACTCTGGATACGTTGCGTCaa   <  1:244765/139‑1 (MQ=255)                                                                                                                              ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAAt  <  1:37005/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   TGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAAT  >  NC_000913/892736‑892999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑