Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	84,690	C→T	A108V (GCG→GTG)	leuO →	global transcription factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	84,690	0	C	T	100.0%	207.9 / NA	65	A108V (GCG→GTG)	leuO	global transcription factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/33);  total (32/33)

TGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTCAAGTCTTCATTAAATCAGAACAC  >  NC_000913/84555‑84825                                                                                                                                        |                                                                                                                                        tGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAg                                                                                                                                      <  2:179865/139‑1 (MQ=255) tGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAg                                                                                                                                      >  2:582095/1‑139 (MQ=255)     tAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGt                                                                                                                                  <  2:920175/139‑1 (MQ=255)         gACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAAc                                                                                                                              >  2:265864/1‑139 (MQ=255)          aCGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACg                                                                                                                             <  2:184126/139‑1 (MQ=255)          aCGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACg                                                                                                                             <  1:81979/139‑1 (MQ=255)           cGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACgt                                                                                                                            >  1:983185/1‑139 (MQ=255)             aGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGc                                                                                                                                     >  2:971060/1‑128 (MQ=255)             aGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGc                                                                                                                                     <  1:971060/128‑1 (MQ=255)             aGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCa                                                                                                                                    >  1:355259/1‑129 (MQ=255)             aGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCa                                                                                                                                    <  2:355259/129‑1 (MQ=255)             aGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACgtgt                                                                                                                          >  1:922517/1‑139 (MQ=255)                tttttGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTAtt                                                                                                                       <  1:582095/139‑1 (MQ=255)                                 cGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCa                                                                                                      <  2:922517/139‑1 (MQ=255)                                 cGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCa                                                                                                      <  2:465050/139‑1 (MQ=255)                                    ggTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGcc                                                                                                   >  2:744680/1‑139 (MQ=255)                                     gTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGTTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACg                                                                                                                             <  1:648493/112‑1 (MQ=255)                                     gTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCCGTGAACg                                                                                                                             >  2:648493/1‑112 (MQ=255)                                            aCCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGAc                                                                                           >  2:253913/1‑139 (MQ=255)                                             ccGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAg                                                                                                                                      <  1:260320/95‑1 (MQ=255)                                             ccGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAg                                                                                                                                      >  2:260320/1‑95 (MQ=255)                                               gACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGc                                                                                        >  2:94429/1‑139 (MQ=255)                                                aCTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACa                                                                                          >  2:47832/1‑136 (MQ=255)                                                aCTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACa                                                                                          <  1:47832/136‑1 (MQ=255)                                                aCTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCa                                                                                       >  2:7658/1‑139 (MQ=255)                                                aCTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCa                                                                                       >  1:946247/1‑139 (MQ=255)                                                  tGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCAtt                                                                                     >  1:194146/1‑139 (MQ=255)                                                  tGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCAtt                                                                                     >  1:341014/1‑139 (MQ=255)                                                    cTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCt                                                                                   >  1:781263/1‑139 (MQ=255)                                                          cATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTc                                                                             <  2:781263/139‑1 (MQ=255)                                                               cAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACgt                                                                                                                            <  1:162754/87‑1 (MQ=255)                                                               cAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACgt                                                                                                                            >  2:162754/1‑87 (MQ=255)                                                                   tttttGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTta                                                                    <  1:94429/139‑1 (MQ=255)                                                                            cAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACAt                                                           >  2:866738/1‑139 (MQ=255)                                                                                       gCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTg                                                >  2:263735/1‑139 (MQ=255)                                                                                        cATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTgc                                               <  2:758195/139‑1 (MQ=255)                                                                                        cATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTgc                                               <  2:341014/139‑1 (MQ=255)                                                                                         aTTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTgcg                                              <  2:336016/139‑1 (MQ=255)                                                                                           tGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAg                                                                                                                                   <  1:290264/52‑1 (MQ=255)                                                                                           tGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAg                                                                                                                                   >  2:290264/1‑52 (MQ=255)                                                                                           tGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGcc                                            >  2:842581/1‑139 (MQ=255)                                                                                           tGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGcc                                            <  1:263735/139‑1 (MQ=255)                                                                                            gCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGcc                                                                                                   >  1:589965/1‑83 (MQ=255)                                                                                            gCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGcc                                                                                                   <  2:589965/83‑1 (MQ=255)                                                                                                  aGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCa                                                                                                      <  1:934144/74‑1 (MQ=255)                                                                                                  aGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCa                                                                                                      >  2:934144/1‑74 (MQ=255)                                                                                                  aGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCa                                                                          <  2:86084/102‑1 (MQ=255)                                                                                                  aGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCa                                                                          >  1:86084/1‑102 (MQ=255)                                                                                                  aGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAAtata                                     <  1:995576/139‑1 (MQ=255)                                                                                                   gTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATAc                                    <  1:842581/139‑1 (MQ=255)                                                                                                   gTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATAc                                    <  1:253913/139‑1 (MQ=255)                                                                                                           tGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCAATCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACatgtt                               <  1:255185/136‑1 (MQ=255)                                                                                                           tGAATTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTATATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACatgtt                               >  2:255185/1‑136 (MQ=255)                                                                                                              aTTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCg                                                                            >  1:124771/1‑88 (MQ=255)                                                                                                              aTTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCg                                                                            <  2:124771/88‑1 (MQ=255)                                                                                                              aTTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATAc                                    <  1:333030/128‑1 (MQ=255)                                                                                                              aTTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATAc                                    >  2:333030/1‑128 (MQ=255)                                                                                                              aTTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTc                         >  1:491278/1‑139 (MQ=255)                                                                                                              aTTGCCTGGTTCAGGTTTTGAACCCGTGAGCAGTGAACGTGAATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTc                         >  1:412636/1‑139 (MQ=255)                                                                                                                            gTTTTGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTCAAGTCTTCATTaaa           >  2:853091/1‑139 (MQ=255)                                                                                                                             ttttGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCTTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTCAAGTCTTCATTAAAt          <  1:866738/139‑1 (MQ=255)                                                                                                                             ttttGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTCAAGTCTTCATTAAAt          <  2:983185/139‑1 (MQ=255)                                                                                                                                tGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTCa                        >  1:326559/1‑122 (MQ=255)                                                                                                                                tGAACCCGTGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTCa                        <  2:326559/122‑1 (MQ=255)                                                                                                                                     ccGTGAGCAGTGAACGTGAATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTCAAGTCTTCATTAAATCAGAacac  <  2:412636/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTCAAGTCTTCATTAAATCAGAACAC  >  NC_000913/84555‑84825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑