Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 96,008 G→A *453* (TAG→TAA
V3I (GTT→ATT) 
murF →
mraY →
UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
phospho‑N‑acetylmuramoyl‑pentapeptide transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091396,0080GA100.0% 126.6 / NA 40*453* (TAG→TAA
V3I (GTT→ATT) 
murF
mraY
UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
phospho‑N‑acetylmuramoyl‑pentapeptide transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/19);  total (21/19)

TTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGA  >  NC_000913/95899‑96134
                                                                                                             |                                                                                                                              
ttAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAatat                                                                                                   >  2:490315/1‑139 (MQ=255)
             tGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                      >  2:677182/1‑139 (MQ=255)
                  aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGaa                                                                                                                  >  2:437947/1‑106 (MQ=255)
                  aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGaa                                                                                                                  <  1:437947/106‑1 (MQ=255)
                       cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTc                                                                            >  2:534508/1‑139 (MQ=255)
                            aaTTACGATTTTAGTTAAGGGCTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtg                                                                                                                            >  1:965819/1‑86 (MQ=255)
                            aaTTACGATTTTAGTTAAGGGCTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtg                                                                                                                            <  2:965819/86‑1 (MQ=255)
                                 cGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                                         <  2:994726/100‑1 (MQ=255)
                                 cGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                                         >  1:994726/1‑100 (MQ=255)
                                 cGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTaa                                                                                     <  1:987578/120‑1 (MQ=255)
                                 cGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTaa                                                                                     >  2:987578/1‑120 (MQ=255)
                                        aGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTcgcg                                                           <  1:534508/139‑1 (MQ=255)
                                                   cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                      <  2:264565/101‑1 (MQ=255)
                                                   cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                      >  1:264565/1‑101 (MQ=255)
                                                          tGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCtttt                                                                             >  2:42622/1‑103 (MQ=255)
                                                          tGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCtttt                                                                             <  1:42622/103‑1 (MQ=255)
                                                                  tGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGt                                                                                  >  2:746508/1‑90 (MQ=255)
                                                                  tGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGt                                                                                  <  1:746508/90‑1 (MQ=255)
                                                                    gAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtgg                                                                                                                           >  1:260247/1‑47 (MQ=255)
                                                                    gAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtgg                                                                                                                           <  2:260247/47‑1 (MQ=255)
                                                                       gagGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAt                            <  1:482174/139‑1 (MQ=255)
                                                                          gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTg                                 >  1:334136/1‑131 (MQ=255)
                                                                          gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTg                                 <  2:334136/131‑1 (MQ=255)
                                                                               aCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTc                                                                                 >  1:94161/1‑78 (MQ=255)
                                                                               aCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTc                                                                                 <  2:94161/78‑1 (MQ=255)
                                                                                       tACAGGAGAATGGGACATGTTAATTTGGCTGGCCGATCATTTGGTCAAATATTATTCCGGCTTTAACGTCCTTTCCTATCTGACGTTTTGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc            <  1:490315/139‑1 (MQ=255)
                                                                                       tACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc            <  1:677182/139‑1 (MQ=255)
                                                                                                 tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                                         <  2:223758/36‑1 (MQ=255)
                                                                                                 tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                                         >  1:223758/1‑36 (MQ=255)
                                                                                                 tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGt                                                                                  >  2:893023/1‑59 (MQ=255)
                                                                                                 tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGt                                                                                  <  1:893023/59‑1 (MQ=255)
                                                                                                 tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTc                                                   >  2:164882/1‑90 (MQ=255)
                                                                                                 tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTc                                                   <  1:164882/90‑1 (MQ=255)
                                                                                                 tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGa  >  2:326636/1‑139 (MQ=255)
                                                                                                 tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGa  >  2:303049/1‑139 (MQ=255)
                                                                                                 tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGa  >  1:195573/1‑139 (MQ=255)
                                                                                                      cATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGcc                                                         <  2:769510/79‑1 (MQ=255)
                                                                                                      cATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGcc                                                         >  1:769510/1‑79 (MQ=255)
                                                                                                      cATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc            <  2:445335/124‑1 (MQ=255)
                                                                                                      cATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc            >  1:445335/1‑124 (MQ=255)
                                                                                                             |                                                                                                                              
TTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGA  >  NC_000913/95899‑96134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: