Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,089,570	T→C	E96E (GAA→GAG)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,089,570	0	T	C	100.0%	147.4 / NA	41	E96E (GAA→GAG)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/20);  total (21/20)

CGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCTTCGTCTTCAACGTTATGCCATGAAAT  >  NC_000913/1089440‑1089704                                                                                                                                   |                                                                                                                                       cGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAAt                                                                                                                                <  2:279766/139‑1 (MQ=255) cGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAAt                                                                                                                                <  1:37437/139‑1 (MQ=255) cGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAAt                                                                                                                                <  1:518737/139‑1 (MQ=255)      gCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAg                                                                                                                           <  2:769485/139‑1 (MQ=255)       cccATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGc                                                                                                                          >  2:425896/1‑139 (MQ=255)                cagGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGTGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAg                                                                                                                           <  2:602847/129‑1 (MQ=255)                cagGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAg                                                                                                                           <  2:577209/129‑1 (MQ=255)                cagGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGAc                                                                                                                 >  2:296746/1‑139 (MQ=255)                cagGCCACTGGAAGGCCTGAAGAATTGCGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAg                                                                                                                           >  1:602847/1‑129 (MQ=255)                cagGCCACTGGAAGGCCAGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCGGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAg                                                                                                                           >  1:577209/1‑129 (MQ=255)                               ccTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGtt                                                                                                  >  2:266104/1‑139 (MQ=255)                                  gaagaaTTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGCTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTtctc                                                                                               >  1:219756/1‑139 (MQ=255)                                         tGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGc                                                                                        >  2:514409/1‑139 (MQ=255)                                           ggAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCa                                                                                      >  2:24140/1‑139 (MQ=255)                                             aaGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAAt                                                                                                                                >  1:377008/1‑95 (MQ=255)                                             aaGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAAt                                                                                                                                <  2:377008/95‑1 (MQ=255)                                                aCGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCaa                                                                                 >  2:387575/1‑139 (MQ=255)                                                          aaaaaCTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                                                       <  1:266104/139‑1 (MQ=255)                                                          aaaaaCTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                                                       <  1:855894/139‑1 (MQ=255)                                                          aaaaaCTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGCTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                                                       <  2:219756/139‑1 (MQ=255)                                                                tctGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGc                                                                 >  2:97205/1‑139 (MQ=255)                                                                  tGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTg                                                               >  2:907575/1‑139 (MQ=255)                                                                   ggTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGTGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGTAGTGCTGa                                                              >  2:574405/1‑139 (MQ=255)                                                                       gCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTc                                                          >  1:673275/1‑139 (MQ=255)                                                                         cGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTCCACGCAGTGCTGATGTCCg                                                        <  2:673275/139‑1 (MQ=255)                                                                                  aaGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACAt                                               <  1:387575/139‑1 (MQ=255)                                                                                       aGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                                                       >  1:69764/1‑110 (MQ=255)                                                                                       aGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                                                       <  2:69764/110‑1 (MQ=255)                                                                                              cAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTc                                   >  1:635187/1‑139 (MQ=255)                                                                                                 cTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                                                       >  1:640143/1‑100 (MQ=255)                                                                                                 cTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                                                       <  2:640143/100‑1 (MQ=255)                                                                                                     ttCCGGTAGTGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCt                            <  1:574405/139‑1 (MQ=255)                                                                                                      tCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGAc                                                                                                                 >  1:925664/1‑53 (MQ=255)                                                                                                      tCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGAc                                                                                                                 <  2:925664/53‑1 (MQ=255)                                                                                                           tAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCTTCGTCt                      >  2:61017/1‑139 (MQ=255)                                                                                                            aGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCTTCGTCtt                     <  1:24140/139‑1 (MQ=255)                                                                                                            aGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCTTCGTCtt                     <  1:514409/139‑1 (MQ=255)                                                                                                                           cGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                                                       <  2:851210/74‑1 (MQ=255)                                                                                                                           cGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                                                       >  1:851210/1‑74 (MQ=255)                                                                                                                             aTGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCTTCGTCTTCAACGTTATGCCATGaa    >  2:534729/1‑139 (MQ=255)                                                                                                                               gtgCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCTTCGTCTTCAACGTTATGCCATGAAAt  <  2:635187/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       CGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCTTCGTCTTCAACGTTATGCCATGAAAT  >  NC_000913/1089440‑1089704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑