Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,307	C→T	*47* (TAG→TAA)	yceO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,307	0	C	T	100.0%	110.5 / NA	36	*47* (TAG→TAA)	yceO	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

ATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACG  >  NC_000913/1119173‑1119440                                                                                                                                       |                                                                                                                                      aTCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaaa                                                                                                                                   >  1:162552/1‑139 (MQ=255)     tCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGACTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGTTTAAAGATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATa                                                                                                                               <  2:675792/139‑1 (MQ=255)                      tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                   <  1:285518/134‑1 (MQ=255)                      tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                   >  2:285518/1‑134 (MQ=255)                        tgatgctgagaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATcc                                                                                                            >  1:879847/8‑139 (MQ=255)                        aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                             >  1:131040/1‑122 (MQ=255)                        aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                             <  2:131040/122‑1 (MQ=255)                                    gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCcaac                                                                                                >  2:877299/1‑139 (MQ=255)                                              tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                             <  2:865744/100‑1 (MQ=255)                                              tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                             >  1:865744/1‑100 (MQ=255)                                                   tAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaaaa                                                                                 >  1:41043/1‑139 (MQ=255)                                                        gCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGc                                                                                                    >  2:697377/1‑115 (MQ=255)                                                        gCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGc                                                                                                    <  1:697377/115‑1 (MQ=255)                                                             aataatCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGAACTATAATTGCCAACAATCCCCAGAGAAAAAGACAAAGCa                                                                       >  1:992951/1‑139 (MQ=255)                                                                       ttCTATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATg                                                                >  2:10091/1‑136 (MQ=255)                                                                       ttCTATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATg                                                                <  1:10091/136‑1 (MQ=255)                                                                       ttCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCcaaca                                                                                               >  2:646362/1‑105 (MQ=255)                                                                       ttCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCcaaca                                                                                               <  1:646362/105‑1 (MQ=255)                                                                          aaTTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgcg                                                          <  2:879847/139‑1 (MQ=255)                                                                          aaTTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgcg                                                          <  2:162552/139‑1 (MQ=255)                                                                              tttCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAGTCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAata                                                      <  1:884848/139‑1 (MQ=255)                                                                                tCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtatt                                                    <  2:41043/139‑1 (MQ=255)                                                                                     cTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtga                                               <  2:19933/139‑1 (MQ=255)                                                                                                         atatTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATc                                                                     <  2:269208/97‑1 (MQ=255)                                                                                                         atatTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATc                                                                     >  1:269208/1‑97 (MQ=255)                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaa                                                                           <  2:252102/70‑1 (MQ=255)                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaa                                                                           >  1:252102/1‑70 (MQ=255)                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGa                                                                  >  1:745443/1‑79 (MQ=255)                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGa                                                                  <  2:745443/79‑1 (MQ=255)                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                        <  1:893377/89‑1 (MQ=255)                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                        >  2:893377/1‑89 (MQ=255)                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCg                            <  2:754343/117‑1 (MQ=255)                                                                                                                              tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCg                            >  1:754343/1‑117 (MQ=255)                                                                                                                                cGGAAAATTAAAAATAAAACACATAGATCAGATCCATAAtt                                                                                                      <  2:407306/41‑1 (MQ=255)                                                                                                                                cGGAAAATTAAAAATAAAACACATAGATCAGATCCATAAtt                                                                                                      >  1:407306/1‑41 (MQ=255)                                                                                                                                  gAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACg  >  1:261363/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      ATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACG  >  NC_000913/1119173‑1119440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑