Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,691	G→A	*357* (TAG→TAA)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,691	0	G	A	100.0%	94.6 / NA	30	*357* (TAG→TAA)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/20);  total (10/20)

GGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTA  >  NC_000913/1148562‑1148763                                                                                                                                  |                                                                         ggTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCt                                                                 <  2:538165/139‑1 (MQ=255)              cAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCg                                                    <  2:924603/139‑1 (MQ=255)              cAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCg                                                    <  2:366807/139‑1 (MQ=255)                            ggTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGa                                      <  2:355006/139‑1 (MQ=255)                               gCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACgg                                   >  1:555847/1‑139 (MQ=255)                                 aGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGta                                 <  1:278382/139‑1 (MQ=255)                                        aGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGAc                                     >  1:200415/1‑128 (MQ=255)                                        aGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGAc                                     <  2:200415/128‑1 (MQ=255)                                               cAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCa                                                  >  1:676577/1‑108 (MQ=255)                                               cAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCa                                                  <  2:676577/108‑1 (MQ=255)                                                aGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTg                  <  2:555847/139‑1 (MQ=255)                                                 gCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGa                 <  1:916316/139‑1 (MQ=255)                                                 gCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGa                 <  1:249525/139‑1 (MQ=255)                                                      ttGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg            <  2:877845/139‑1 (MQ=255)                                                      ttGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg            <  1:308101/139‑1 (MQ=255)                                                                ccTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGta  <  2:232768/139‑1 (MQ=255)                                                                   ggAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg                                         <  2:934665/97‑1 (MQ=255)                                                                   ggAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg                                         >  1:934665/1‑97 (MQ=255)                                                                           tataCCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACa      <  1:355378/124‑1 (MQ=255)                                                                           tataCCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACa      >  2:355378/1‑124 (MQ=255)                                                                                 cAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACt               <  2:1007189/109‑1 (MQ=255)                                                                                 cAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACt               >  1:1007189/1‑109 (MQ=255)                                                                                   gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGt                                        >  1:75686/1‑82 (MQ=255)                                                                                   gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGt                                        <  2:75686/82‑1 (MQ=255)                                                                                   gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGa                                      <  2:216025/84‑1 (MQ=255)                                                                                   gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGa                                      >  1:216025/1‑84 (MQ=255)                                                                                   gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACt               >  2:719759/1‑107 (MQ=255)                                                                                   gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACt               <  1:719759/107‑1 (MQ=255)                                                                                                                    ggAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGtata                               >  2:607928/1‑58 (MQ=255)                                                                                                                    ggAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGtata                               <  1:607928/58‑1 (MQ=255)                                                                                                                                  |                                                                         GGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTA  >  NC_000913/1148562‑1148763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑