Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,038	G→A	A325T (GCT→ACT)	dhaR →	dhaKLM operon transcription activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,038	0	G	A	100.0%	51.1 / NA	18	A325T (GCT→ACT)	dhaR	dhaKLM operon transcription activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

TAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGC  >  NC_000913/1251929‑1252165                                                                                                              |                                                                                                                                tAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAAcc                                                                                                    >  1:884289/1‑139 (MQ=255)                  cGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCAttt                                                                                   >  2:561546/1‑139 (MQ=255)                   gCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCAtttt                                                                                  <  2:535692/139‑1 (MQ=255)                     aGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTgg                                                                                <  1:520931/139‑1 (MQ=255)                                          tgCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAg                                                           <  1:561546/139‑1 (MQ=255)                                                         aaCAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCt                                            <  2:994263/139‑1 (MQ=255)                                                           cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCttt                                          >  1:950469/1‑139 (MQ=255)                                                                gCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCaaa                                                                                                      >  2:756736/1‑74 (MQ=255)                                                                gCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCaaa                                                                                                      <  1:756736/74‑1 (MQ=255)                                                                   gCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGagaa                                  >  1:125817/1‑139 (MQ=255)                                                                                aGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCa                     <  2:884289/139‑1 (MQ=255)                                                                                          gAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCt           <  1:961190/139‑1 (MQ=255)                                                                                            aaaGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCcgccgc                                                                                              >  2:365312/1‑54 (MQ=255)                                                                                            aaaGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCcgccgc                                                                                              <  1:365312/54‑1 (MQ=255)                                                                                            aaaGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTaa         >  2:179492/1‑139 (MQ=255)                                                                                            aaaGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTaa         >  1:483249/1‑139 (MQ=255)                                                                                                  aGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAgg   <  1:179492/139‑1 (MQ=255)                                                                                                   gCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGc  <  2:950469/139‑1 (MQ=255)                                                                                                              |                                                                                                                                TAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGC  >  NC_000913/1251929‑1252165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑