Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,309,016 C→T *418* (TAG→TAA)  kch ← voltage‑gated potassium channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,309,0160CT100.0% 159.7 / NA 49*418* (TAG→TAA) kchvoltage‑gated potassium channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/22);  total (27/22)

TCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCAC  >  NC_000913/1308891‑1309134
                                                                                                                             |                                                                                                                      
tCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCg                                                                                                           <  2:766778/139‑1 (MQ=255)
          cAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACt                                                                                                 >  2:847383/1‑139 (MQ=255)
            ggAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCg                                                                                                           <  1:314522/127‑1 (MQ=255)
            ggAAGTGCATCCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCg                                                                                                           >  2:314522/1‑127 (MQ=255)
                   cATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCttt                                                                                        >  2:940871/1‑139 (MQ=255)
                   cATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCttt                                                                                        <  1:993405/139‑1 (MQ=255)
                       cTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGtt                                                                                    >  2:1002500/1‑139 (MQ=255)
                        tAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGttt                                                                                   <  1:847383/139‑1 (MQ=255)
                        tAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGttt                                                                                   >  1:228572/1‑139 (MQ=255)
                          aacaaacaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCt                                                                                 <  1:1002500/139‑1 (MQ=255)
                           acaaacaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGAt                                                                                                         >  2:163476/1‑114 (MQ=255)
                           acaaacaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGAt                                                                                                         <  1:163476/114‑1 (MQ=255)
                               acaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                             <  2:525471/106‑1 (MQ=255)
                               acaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                             >  1:525471/1‑106 (MQ=255)
                                   cGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCg                                                                        >  1:866691/1‑139 (MQ=255)
                                        cTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATc                                                                   <  2:228572/139‑1 (MQ=255)
                                                                   cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                        >  1:334458/1‑139 (MQ=255)
                                                                     aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCtt                                                                                         <  2:409357/88‑1 (MQ=255)
                                                                     aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCtt                                                                                         >  1:409357/1‑88 (MQ=255)
                                                                     aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                      >  2:820204/1‑139 (MQ=255)
                                                                     aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                      >  1:950642/1‑139 (MQ=255)
                                                                     aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                      >  2:180017/1‑139 (MQ=255)
                                                                             tGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGaa                              <  2:866691/139‑1 (MQ=255)
                                                                              gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGttt                                                                                   >  1:426338/1‑85 (MQ=255)
                                                                              gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGttt                                                                                   <  2:426338/85‑1 (MQ=255)
                                                                              gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGAc                                                  <  1:47823/118‑1 (MQ=255)
                                                                              gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGAc                                                  >  2:47823/1‑118 (MQ=255)
                                                                              gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACAtt                                 <  2:598741/135‑1 (MQ=255)
                                                                              gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACAtt                                 >  1:598741/1‑135 (MQ=255)
                                                                              gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGaaa                             >  2:936423/1‑139 (MQ=255)
                                                                              gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGaaa                             >  2:997556/1‑139 (MQ=255)
                                                                              gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGaaa                             <  1:936423/139‑1 (MQ=255)
                                                                                gCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTg                                                                                                                <  2:409407/54‑1 (MQ=255)
                                                                                gCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTg                                                                                                                >  1:409407/1‑54 (MQ=255)
                                                                                  aCCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                             >  2:72072/1‑55 (MQ=255)
                                                                                  aCCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                             <  1:72072/55‑1 (MQ=255)
                                                                                         cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCt                                                                                                      <  2:3594/55‑1 (MQ=255)
                                                                                         cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCt                                                                                                      >  1:3594/1‑55 (MQ=255)
                                                                                              cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCattatt             >  2:475241/1‑139 (MQ=255)
                                                                                              cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCattatt             >  2:75019/1‑139 (MQ=255)
                                                                                               ccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCAttatta            <  2:334458/139‑1 (MQ=255)
                                                                                                                ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCa                                                                                             >  2:385555/1‑41 (MQ=255)
                                                                                                                ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCa                                                                                             <  1:385555/41‑1 (MQ=255)
                                                                                                                 cGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGC‑TTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                        >  2:779416/1‑92 (MQ=255)
                                                                                                                 cGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGC‑TTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                        <  1:779416/92‑1 (MQ=255)
                                                                                                                      ttAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                          <  2:139857/38‑1 (MQ=255)
                                                                                                                      ttAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                          >  1:139857/1‑38 (MQ=255)
                                                                                                                      ttAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCAc  <  1:979265/126‑1 (MQ=255)
                                                                                                                      ttAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCAc  >  2:979265/1‑126 (MQ=255)
                                                                                                                             |                                                                                                                      
TCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCAC  >  NC_000913/1308891‑1309134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑